Anti-PPAR delta antibody (ab23673)
Key features and details
- Rabbit polyclonal to PPAR delta
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PPAR delta antibody
See all PPAR delta primary antibodies -
Description
Rabbit polyclonal to PPAR delta -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Chicken
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Immunogen
Synthetic peptide:
SSSYTDLSRSSSPPSL
, corresponding to amino acids 39 - 54 of Human PPAR delta .
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: BSA, Tris buffered saline, ddH20
Contains 5 mg/ml BSA. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PPAR delta antibody (ab23673) This image is courtesy of an anonymous AbreviewAnti-PPAR delta antibody (ab23673) at 1/1000 dilution + Rat brain tissue lysate at 100 µg
Secondary
HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-PPAR delta antibody (ab23673)Immunofluorescent staining of HT-29 cells (ab3952) (nuclear staining) using ab23673.
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Western blot - Anti-PPAR delta antibody (ab23673)All lanes : Anti-PPAR delta antibody (ab23673)
Lane 1 : Human cerebral cortex (30ug)
Lane 2 : Murine liver (30ug)
Predicted band size: 50 kDa
ab23673 recognises PPAR delta at 50kDa from human samples. An additional smaller size of PPAR delta (~40kDa) is also detected in certain murine tissues.
