Anti-LRRTM1 antibody (ab106414)
Key features and details
- Rabbit polyclonal to LRRTM1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-LRRTM1 antibody -
Description
Rabbit polyclonal to LRRTM1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species sELISA MouseWB Mouse
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Immunogen
Synthetic peptide corresponding to Human LRRTM1 aa 500 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab127971) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse Forebrain; Mouse Hippocampus; Mouse Brain P0; Mouse Olfactory Bulb.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species sELISA MouseWB MouseAll applications RatCowDogHumanPigMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB 1/250. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).Notes WB
1/250. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).Target
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Function
May play a role during the development of specific forebrain structures by influencing neuronal differentiation and connectivity, with a possible role in intracellular trafficking within axons. -
Tissue specificity
Predominantly expression in forebrain regions including thalamus and cerebral cortex. -
Sequence similarities
Belongs to the LRRTM family.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 347730 Human
- Entrez Gene: 74342 Mouse
- Entrez Gene: 679668 Rat
- Omim: 610867 Human
- SwissProt: A1A4H9 Cow
- SwissProt: Q86UE6 Human
- SwissProt: Q8K377 Mouse
- Unigene: 591580 Human
see all -
Alternative names
- 4632401D06Rik antibody
- AW125451 antibody
- FLJ32082 antibody
see all
Images
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Western blot - Anti-LRRTM1 antibody (ab106414)All lanes : Anti-LRRTM1 antibody (ab106414) at 1/250 dilution
Lane 1 : Forebrain (Mouse) Tissue Lysate
Lane 2 : Mouse Hippocampus Tissue Lysate
Lane 3 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 4 : Mouse Olfactory Bulb Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 30 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
LRRTM1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
References (0)
ab106414 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-LRRTM1 antibody (ab106414)All lanes : Anti-LRRTM1 antibody (ab106414) at 1/250 dilution
Lane 1 : Forebrain (Mouse) Tissue Lysate
Lane 2 : Mouse Hippocampus Tissue Lysate
Lane 3 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 4 : Mouse Olfactory Bulb Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 30 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
LRRTM1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
