Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸ 10 µl 134 016 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP2567Y] to NCAM1
Suitable for: WB, IP, Flow Cyt, ICC/IF, IHC-P
Reacts with: Human

Product name

Anti-NCAM1 antibody [EP2567Y]
See all NCAM1 primary antibodies
Description

Rabbit monoclonal [EP2567Y] to NCAM1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human NCAM1 aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
Positive control
- WB: SH-SY5Y cell lysate. ICC/IF: SH-SY5Y cells. Flow Cyt: SH-SY5Y cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP2567Y
Isotype

IgG
Research areas

Flow Cytometry - Anti-NCAM1 antibody [EP2567Y] (ab75813)

Overlay histogram showing SH-SY5Y cells stained with ab75813 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75813, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EP2567Y] (ab75813)

ab75813 at 1:1000 staining NCAM1 antibody in human astrocytoma tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling NCAM1 with ab75813 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Positive staining on tumor cells of human astrocytoma is observed. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] (ab75813)

ab75813 at 1/100 dilution staining NCAM in SH-SY5Y cells by Immunofluorescence.
Immunocytochemistry/ Immunofluorescence - Anti-NCAM1 antibody [EP2567Y] (ab75813) This image is courtesy of an anonymous Abreview

ab75813 staining NCAM in human adipose cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 10% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A Cy3^®-conjugated donkey anti-rabbit IgG polyclonal (1/800) was used as the secondary antibody.
See Abreview
Western blot - Anti-NCAM1 antibody [EP2567Y] (ab75813)

Anti-NCAM1 antibody [EP2567Y] (ab75813) at 1/100000 dilution + SH-SY5Y cell
lysate at 10 µg

Secondary
goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 93 kDa
Observed band size: 180 kDa
why is the actual band size different from the predicted?
Anti-NCAM1 antibody [EP2567Y] (ab75813)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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