Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NRP2 antibody [EPR23808-72] (ab273584)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23808-72] to NRP2
  • Suitable for: IP, WB, Flow Cyt, ICC/IF, Immunomicroscopy
  • Reacts with: Human

Overview

  • Product name

    Anti-NRP2 antibody [EPR23808-72]
    See all NRP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23808-72] to NRP2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U-87 MG whole cell lysate. ICC/IF: U-87 MG cells. Flow Cyt: U-87 MG cells. IP: U-87 MG whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-NRP2 antibody [EPR23808-72] (ab273584)
    Immunocytochemistry/ Immunofluorescence - Anti-NRP2 antibody [EPR23808-72] (ab273584)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-87 MG cells labelling NRP2 with ab273584 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in U-87 MG cell line. Negative control: MCF7 (PMID:18818766). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/202 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-NRP2 antibody [EPR23808-72] (ab273584)
    Flow Cytometry - Anti-NRP2 antibody [EPR23808-72] (ab273584)

    Flow cytometric analysis of MCF7 (Human breast adenocarcinoma epithelial cell, Left) / U-87 MG (Human glioblastoma-astrocytoma epithelial cell, Right) cells labelling NRP2 with ab273584 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: MCF7.

    Gated on viable cells.

  • Western blot - Anti-NRP2 antibody [EPR23808-72] (ab273584)
    Western blot - Anti-NRP2 antibody [EPR23808-72] (ab273584)
    All lanes : Anti-NRP2 antibody [EPR23808-72] (ab273584) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 105 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:18394556, 26156437).

    Negative control: MCF 7 (PMID:17912247).

    Exposure time: 3 minutes.

  • Immunoprecipitation - Anti-NRP2 antibody [EPR23808-72] (ab273584)
    Immunoprecipitation - Anti-NRP2 antibody [EPR23808-72] (ab273584)

    NRP2 was immunoprecipitated from 0.35 mg U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate with ab273584 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273584 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate 10 ug

    Lane 2: ab273584 IP in U-87 MG whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273584 in U-87 MG whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

     

  • Anti-NRP2 antibody [EPR23808-72] (ab273584)
    Anti-NRP2 antibody [EPR23808-72] (ab273584)

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