Anti-GAP43 antibody [EPR5031] (ab134075)
![Anti-GAP43 antibody [EPR5031] (ab134075)](https://www.abcam.com/ps/products/134/ab134075/Images/ab134075-148212-anti-gap43-antibody-epr5031-western-blot.jpg "Anti-GAP43 antibody [EPR5031] (ab134075)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5031] to GAP43
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-GAP43 antibody [EPR5031]
See all GAP43 primary antibodies -
Description
Rabbit monoclonal [EPR5031] to GAP43 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human GAP43 aa 50-150. The exact sequence is proprietary.
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Positive control
- SH-SY5Y and Human brain lysates; Human glioma tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5031 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GAP43 antibody [EPR5031] (ab134075)All lanes : Anti-GAP43 antibody [EPR5031] (ab134075) at 1/1000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : Human brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution
Predicted band size: 24 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody [EPR5031] (ab134075)](https://www.abcam.com/ps/products/134/ab134075/Images/ab134075-148211-anti-gap43-antibody-epr5031-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAP43 antibody [EPR5031] (ab134075)Immunohistochemical analysis of paraffin embedded Human glioma tissue labelling GAP43 with ab134075 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-GAP43 antibody [EPR5031] (ab134075)](https://www.abcam.com/ps/products/134/ab134075/Images/ab134075-3-benefits-of-recombinant-antibodies.png)
Anti-GAP43 antibody [EPR5031] (ab134075)
