Anti-GAP43 antibody - Neuronal Marker (ab16053)
Key features and details
- Rabbit polyclonal to GAP43 - Neuronal Marker
- Suitable for: WB, IHC (PFA fixed)
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-GAP43 antibody - Neuronal Marker
See all GAP43 primary antibodies -
Description
Rabbit polyclonal to GAP43 - Neuronal Marker -
Host species
Rabbit -
Specificity
This antibody does not perform well in IHC-P (abreview 21090, 21089) -
Tested applications
Suitable for: WB, IHC (PFA fixed)more details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human, Chimpanzee
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Immunogen
Synthetic peptide corresponding to Rat GAP43 aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab16379)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab16053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 43-53 kDa (predicted molecular weight: 43-53 kDa). IHC (PFA fixed) Use at an assay dependent concentration. Application notesIs unsuitable for IHC-P.Target
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Function
This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. -
Sequence similarities
Belongs to the neuromodulin family.
Contains 1 IQ domain. -
Post-translational
modificationsPhosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. -
Cellular localization
Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes. - Information by UniProt
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Database links
- Entrez Gene: 460599 Chimpanzee
- Entrez Gene: 2596 Human
- Entrez Gene: 14432 Mouse
- Entrez Gene: 29423 Rat
- GenBank: NP_002036 Human
- Omim: 162060 Human
- SwissProt: P17677 Human
- SwissProt: P06837 Mouse
see all -
Alternative names
- Axonal membrane protein GAP 43 antibody
- Axonal membrane protein GAP-43 antibody
- B 50 antibody
see all
Images
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Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)All lanes : Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml
Lane 1 : spinal cord chung rat 1
Lane 2 : spinal cord chung rat 2
Lane 3 : spinal cord chung rat 3
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Dako Goat polyclonal to Rabbit IgG (HRP) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 43-53 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 90 kDa (possible multimer) -
Immunohistochemistry (PFA fixed) - Anti-GAP43 antibody - Neuronal Marker (ab16053)This image is courtesy of Sophie Pezet, Univ London Kings Coll, United KingdomImmunofluorescent staining obtained with ab16053 Rabbit polyclonal to GAP43 in rat dorsal root ganglion, 2 weeks after a spinal nerve injury. The staining is localised in the cytoplasm of some lesioned neurons (arrow in B) and in many axons (arrow heads in B). Image B (X40 objective) is a higher magnification of A (X20). The staining disappeared completely when the antibody was pre-incubated with the immunising (blocking) peptide (ab16379).
Protocol details: Rats were intracardially perfused with 4% paraformaldehyde, DRG tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. IHC free floating was performed on the fixed, frozen and cut (20µm sections) rat tissue. Primary antibody ab16053 was incubated overnight at toom temperature at 1/300. Secondary antibody Alexa fluor 488 was incubated at 1/1000 for 2 hours at room temperature.
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Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43-53 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Protocols
References (40)
ab16053 has been referenced in 40 publications.
- van der Merwe Y et al. Matrix-bound nanovesicles prevent ischemia-induced retinal ganglion cell axon degeneration and death and preserve visual function. Sci Rep 9:3482 (2019). PubMed: 30837658
- Li L et al. Ascorbic Acid Facilitates Neural Regeneration After Sciatic Nerve Crush Injury. Front Cell Neurosci 13:108 (2019). PubMed: 30949031
- Li J et al. TGN-020 alleviates edema and inhibits astrocyte activation and glial scar formation after spinal cord compression injury in rats. Life Sci 222:148-157 (2019). PubMed: 30851336
- Alrawashdeh W et al. Perineural invasion in pancreatic cancer: proteomic analysis and in vitro modelling. Mol Oncol 13:1075-1091 (2019). PubMed: 30690892
- Liu Y et al. Nogo-A/Pir-B/TrkB Signaling Pathway Activation Inhibits Neuronal Survival and Axonal Regeneration After Experimental Intracerebral Hemorrhage in Rats. J Mol Neurosci 69:360-370 (2019). PubMed: 31286407
Images
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Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)All lanes : Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml
Lane 1 : spinal cord chung rat 1
Lane 2 : spinal cord chung rat 2
Lane 3 : spinal cord chung rat 3
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Dako Goat polyclonal to Rabbit IgG (HRP) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 43-53 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 90 kDa (possible multimer)
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Immunohistochemistry (PFA fixed) - Anti-GAP43 antibody - Neuronal Marker (ab16053) This image is courtesy of Sophie Pezet, Univ London Kings Coll, United Kingdom
Immunofluorescent staining obtained with ab16053 Rabbit polyclonal to GAP43 in rat dorsal root ganglion, 2 weeks after a spinal nerve injury. The staining is localised in the cytoplasm of some lesioned neurons (arrow in B) and in many axons (arrow heads in B). Image B (X40 objective) is a higher magnification of A (X20). The staining disappeared completely when the antibody was pre-incubated with the immunising (blocking) peptide (ab16379).
Protocol details: Rats were intracardially perfused with 4% paraformaldehyde, DRG tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. IHC free floating was performed on the fixed, frozen and cut (20µm sections) rat tissue. Primary antibody ab16053 was incubated overnight at toom temperature at 1/300. Secondary antibody Alexa fluor 488 was incubated at 1/1000 for 2 hours at room temperature.
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Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43-53 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
