Anti-LIM kinase 1 + 2 antibody (ab51200)
Key features and details
- Rabbit polyclonal to LIM kinase 1 + 2
- Suitable for: IHC-P, WB
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-LIM kinase 1 + 2 antibody -
Description
Rabbit polyclonal to LIM kinase 1 + 2 -
Host species
Rabbit -
Specificity
Detects endogenous levels of total LIMK1 and LIMK2 proteins. Ab51200 is produced using a phospho peptide immunogen. The phospho-specific antibody is removed by affinity column and pan antibody was purified by non-phospho-peptide columns. This antibody will therefore react with both phosphorylated and non phosphorylated forms of LIMK1 and LIMK2 proteins. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB African green monkey
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Immunogen
Synthesized non-phosphopeptide derived from human LIMK1 and LIMK2 around the phosphorylation site of threonine 508/505 (R-Y-TP-V-V).
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Positive control
- Human breast carcinoma tissue Extracts from cos7 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM kinase 1 + 2 antibody (ab51200)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab51200 LIMK1/2 antibody.
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Western blot - Anti-LIM kinase 1 + 2 antibody (ab51200)All lanes : Anti-LIM kinase 1 + 2 antibody (ab51200)
Lane 1 : Extracts from cos7 cells
Lane 2 : Extracts from cos7 cells with Immunizing peptide
Predicted band size: 72 , 73 kDa
Observed band size: ~70 kDa why is the actual band size different from the predicted?