Anti-SPTBN2 antibody [ABM5A16] (ab228447)
Key features and details
- Mouse monoclonal [ABM5A16] to SPTBN2
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-SPTBN2 antibody [ABM5A16]
See all SPTBN2 primary antibodies -
Description
Mouse monoclonal [ABM5A16] to SPTBN2 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human SPTBN2 aa 1280-1450.
Database link: O15020 -
Positive control
- WB: Human brain and kidney lysates. Flow Cyt: A431 and HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
ABM5A16 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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All lanes : Anti-SPTBN2 antibody [ABM5A16] (ab228447) at 0.1 µg/ml
Lane 1 : Human kidney lysate
Lane 2 : Human brain lysate
Predicted band size: 271, 268 kDa
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Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cells, labeling SPTBN2 with ab228447 at 2 µg/106 cells (red) compared to an isotype control (green). Goat anti-mouse PE conjugate was used as secondary antibody.
Cells were fixed with 4% paraformaldehyde for 10 minutes and washed with PBS by centrifuging at 1100 for 5 minutes followed by permeabilization for 20 minutes and washed again. The cells were incubated with primary antibody for 45 minutes and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 minutes. Data acquisition was done after washing twice with PBS.
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Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling SPTBN2 with ab228447 at 2 µg/106 cells (red) compared to an isotype control (green). Goat anti-mouse PE conjugate was used as secondary antibody.
Cells were fixed with 4% paraformaldehyde for 10 minutes and washed with PBS by centrifuging at 1100 for 5 minutes followed by permeabilization for 20 minutes and washed again. The cells were incubated with primary antibody for 45 minutes and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 minutes. Data acquisition was done after washing twice with PBS.