Anti-SPTBN2 antibody (ab264178)
Key features and details
- Rabbit polyclonal to SPTBN2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPTBN2 antibody
See all SPTBN2 primary antibodies -
Description
Rabbit polyclonal to SPTBN2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human SPTBN2 aa 2340-2390. The exact sequence is proprietary.
Database link: O15020 -
Positive control
- WB: HeLa and HEK-293T whole cell lysate; IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-SPTBN2 antibody (ab264178)
SPTBN2 was immunoprecipitated from 1mg of HeLa cells (20% of IP loaded) using ab264178 at 6 µg per reaction. Western blot was performed on the immunprecipitate using ab264178 at 0.4 μg/ml.
Lane 1: ab264178 in HeLa whole cell lysate
Lane 2: Control IgG in HeLa whole cell lysate
Detected with chemilimuniscence with exposure time of 3 minutes.
Cells prepared using NETN lysis buffer.
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Western blot - Anti-SPTBN2 antibody (ab264178)All lanes : Anti-SPTBN2 antibody (ab264178) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T whole cell lysate at 50 µg
Lane 4 : HEK-293T whole cell lysate at 15 µg
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Developed using the ECL technique.
Exposure time: 3 minutes4 - 8% SDS-PAGE.
Cells prepared using NETN lysis buffer.
