Anti-Sarcomeric Alpha Actinin antibody (ab227216)
Key features and details
- Rabbit polyclonal to Sarcomeric Alpha Actinin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Sarcomeric Alpha Actinin antibody
See all Sarcomeric Alpha Actinin primary antibodies -
Description
Rabbit polyclonal to Sarcomeric Alpha Actinin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Pig -
Immunogen
Recombinant fragment within Human Sarcomeric Alpha Actinin (internal sequence). The exact sequence is proprietary.
Database link: P35609 -
Positive control
- WB: A431, NCI-H1299 and NIH/3T3 whole cell lysates; Rat heart tissue extract. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin-embedded human breast carcinoma tissue stained for Sarcomeric Alpha Actinin using ab227216 at 1/250 dilution in immunohistochemical analysis.
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Anti-Sarcomeric Alpha Actinin antibody (ab227216) at 1/3000 dilution + NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 104 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab227216) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 104 kDa7.5% SDS-PAGE gel.
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Anti-Sarcomeric Alpha Actinin antibody (ab227216) at 1/5000 dilution + Rat heart tissue extract at 50 µg
Predicted band size: 104 kDa7.5% SDS-PAGE gel.
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PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Sarcomeric Alpha Actinin (green) using ab227216 at 1/200 dilution in ICC/IF.
Right-hand panel: Merged with DNA probe.