Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)
Key features and details
- Mouse monoclonal [EA-53] to Sarcomeric Alpha Actinin
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Sarcomeric Alpha Actinin antibody [EA-53]
See all Sarcomeric Alpha Actinin primary antibodies -
Description
Mouse monoclonal [EA-53] to Sarcomeric Alpha Actinin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Rat -
Immunogen
Full length native protein (purified) corresponding to Rabbit Sarcomeric Alpha Actinin. Purified rabbit skeletal α-actinin.
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Positive control
- WB: Mouse and rat skeletal muscle and rat heart tissue lysates. IHC-P: Human skeletal muscle tissue.
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General notes
This product was changed from ascites to tissue culture supernatant on 9th September 2019. Lot numbers higher than GR3258034 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Proprietary Purification -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
EA-53 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
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Lane 1 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/500 dilution
Lane 2 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/1000 dilution
Lane 3 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/2000 dilution
Lane 4 : No antibody control
All lanes : Rat skeletal muscle extract
Secondary
All lanes : Goat Anti-Mouse IgG (Fab) (HRP) at 1/4000 dilution
Predicted band size: 103 kDa
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Immunohistochemistry formalin-fixed, paraffin-embedded human tongue labeling Alpha Actinin with ab9465 at 12 μg/mL.