Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 117 264 ₸

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Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6375] to Cofilin - Loading Control
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Cofilin antibody [EPR6375] - Loading Control
See all Cofilin primary antibodies
Description

Rabbit monoclonal [EPR6375] to Cofilin - Loading Control
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human Cofilin aa 1-100. The exact sequence is proprietary.
Positive control
- 293T and Caco-2 cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6375
Isotype

IgG
Research areas

Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)

All lanes : Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution (purified)

Lane 1 : NIH/3T3 cell lysate
Lane 2 : C6 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 19 kDa
Observed band size: 19 kDa

Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)

Immunohistochemical staining of paraffin embedded mouse liver with purified ab124979 at a working dilution of 1 in 200. The secondary antibody used is ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)

Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1 % Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lines labeling Cofilin with ab124979 at 1/500 dilution,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

The nuclear counterstain is DAPI (blue).
Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)

Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution (purified) + HEK293 cell lysate at 10 µg

Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 19 kDa
Observed band size: 19 kDa

Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)

Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/5000 dilution (purified) + Caco-2 cell lysate at 10 µg

Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 19 kDa
Observed band size: 19 kDa

Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)

Immunohistochemical staining of paraffin embedded human tonsil with purified ab124979 at a working dilution of 1 in 200. The secondary antibody used is ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)

All lanes : Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution (unpurified)

Lane 1 : 293T cell lysate
Lane 2 : Caco-2 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 19 kDa
Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)

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