Antibodies, Proteins, Kits and Reagents for Life Science

381 945 ₸ Product size

100 µg 381 945 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Mouse monoclonal to Cofilin
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human
Isotype: IgG2a

Product name

Anti-Cofilin antibody
See all Cofilin primary antibodies
Description

Mouse monoclonal to Cofilin
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant full length protein (GST-tag) corresponding to Human Cofilin. AAH11005
Sequence:
MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILE EGKEILVGDVGQTVDDPYATFAKMLPDKDCRYALYDATYETKESKKEDLV FIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEGVKDRCTLA EKLGGSAVISLEGKPL

Database link: P23528
Run BLAST with Run BLAST with
Positive control
- WB: HeLa, PC-12 and NIH/3T3 cell lysate. IHC-P: Human breast cancer tissue. ICC/IF: HeLa cells.
General notes

This product was changed from ascites to tissue culture supernatant on 17/04/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.40
Constituent: PBS
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Isotype

IgG2a
Light chain type

kappa
Research areas

Western blot - Anti-Cofilin antibody (ab54532)

Anti-Cofilin antibody (ab54532) at 1 µg + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 25 µg

Predicted band size: 18 kDa

This image was generated using the ascites version of the product
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody (ab54532)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Cofilin with ab54532 at 1.5 µg/ml in immunohistochemical analysis.

This image was generated using the ascites version of the product.
Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody (ab54532)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cofilin (green) using ab54532 at 10 µg/ml in ICC/IF.

This image was generated using the ascites version of the product.
Western blot - Anti-Cofilin antibody (ab54532)

Anti-Cofilin antibody (ab54532) at 1 µg/ml + NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Predicted band size: 18 kDa

This image was generated using the ascites version of the product.
Western blot - Anti-Cofilin antibody (ab54532)

Anti-Cofilin antibody (ab54532) at 1 µg/ml + PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

Predicted band size: 18 kDa

This image was generated using the ascites version of the product.

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