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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins

Anti-Cofilin antibody (ab227299)

Anti-Cofilin antibody (ab227299)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Cofilin
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Cofilin antibody
    See all Cofilin primary antibodies
  • Description

    Rabbit polyclonal to Cofilin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Cofilin (internal sequence). The exact sequence is proprietary.
    Database link: P23528

  • Positive control

    • WB: HEK-293T whole cell extract; A431, PC-12, Rat2 and NIH/3T3 whole cell lysates. ICC/IF: HeLa cells. IHC-P: HBL435 xenograft tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins
    • Cardiovascular
    • Vasculature
    • Smooth muscle cell (SMC)
    • Cytoskeleton

Images

  • Western blot - Anti-Cofilin antibody (ab227299)
    Western blot - Anti-Cofilin antibody (ab227299)
    All lanes : Anti-Cofilin antibody (ab227299) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : Cofilin shRNA-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 19 kDa



    12% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody (ab227299)
    Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody (ab227299)

    4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cofilin (green) using ab227299 at 1/1000 dilution in ICC/IF. Untreated HeLa cells (left images) and HeLa cells treated with 0.012 N NaOH/PBS for 1 minute (right images). Counterstain: alpha Tubulin was labeled with an anti-alpha Tubulin antibody (red) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody (ab227299)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody (ab227299)

    Paraffin-embedded HBL435 xenograft tissue stained for Cofilin using ab227299 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-Cofilin antibody (ab227299)
    Western blot - Anti-Cofilin antibody (ab227299)
    Anti-Cofilin antibody (ab227299) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 19 kDa



    12% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody (ab227299)
    Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody (ab227299)

    Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cofilin (green) using ab227299 at 1/500 dilution in ICC/IF. Counterstain: alpha Tubulin was labeled with an anti-alpha Tubulin antibody (red) at 1/2000 dilution.

  • Western blot - Anti-Cofilin antibody (ab227299)
    Western blot - Anti-Cofilin antibody (ab227299)
    Anti-Cofilin antibody (ab227299) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 19 kDa



    12% SDS-PAGE gel.

  • Western blot - Anti-Cofilin antibody (ab227299)
    Western blot - Anti-Cofilin antibody (ab227299)
    All lanes : Anti-Cofilin antibody (ab227299) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 19 kDa



    12% SDS-PAGE gel.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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