Anti-Cofilin antibody (ab227299)
Key features and details
- Rabbit polyclonal to Cofilin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cofilin antibody
See all Cofilin primary antibodies -
Description
Rabbit polyclonal to Cofilin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow, Pig, Rhesus monkey -
Immunogen
Recombinant fragment within Human Cofilin (internal sequence). The exact sequence is proprietary.
Database link: P23528 -
Positive control
- WB: HEK-293T whole cell extract; A431, PC-12, Rat2 and NIH/3T3 whole cell lysates. ICC/IF: HeLa cells. IHC-P: HBL435 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cofilin antibody (ab227299) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : Cofilin shRNA-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 19 kDa12% SDS-PAGE gel.
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4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cofilin (green) using ab227299 at 1/1000 dilution in ICC/IF. Untreated HeLa cells (left images) and HeLa cells treated with 0.012 N NaOH/PBS for 1 minute (right images). Counterstain: alpha Tubulin was labeled with an anti-alpha Tubulin antibody (red) at 1/1000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody (ab227299)
Paraffin-embedded HBL435 xenograft tissue stained for Cofilin using ab227299 at 1/500 dilution in immunohistochemical analysis.
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Anti-Cofilin antibody (ab227299) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 19 kDa12% SDS-PAGE gel.
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Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cofilin (green) using ab227299 at 1/500 dilution in ICC/IF. Counterstain: alpha Tubulin was labeled with an anti-alpha Tubulin antibody (red) at 1/2000 dilution.
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Anti-Cofilin antibody (ab227299) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 19 kDa12% SDS-PAGE gel.
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All lanes : Anti-Cofilin antibody (ab227299) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 19 kDa12% SDS-PAGE gel.