Anti-GNB2 antibody [EP3262Y] (ab108504)
![Anti-GNB2 antibody [EP3262Y] (ab108504)](https://www.abcam.com/ps/products/108/ab108504/Images/ab108504-393577-anti-gnb2-antibody-ep3262y-western-blot-human-knockout-lysate.jpg "Anti-GNB2 antibody [EP3262Y] (ab108504)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3262Y] to GNB2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-GNB2 antibody [EP3262Y]
See all GNB2 primary antibodies -
Description
Rabbit monoclonal [EP3262Y] to GNB2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, SH-SY5Y, Jurkat and NIH3T3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP3262Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GNB2 antibody [EP3262Y] (ab108504)All lanes : Anti-GNB2 antibody [EP3262Y] (ab108504) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : GNB2 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab108504 observed at 35 kDa. Red - loading control ab7291 observed at 50 kDa.
ab108504 Anti-GNB2 antibody [EP3262Y] was shown to specifically react with GNB2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266528 (knockout cell lysate ab257453) was used. Wild-type and GNB2 knockout samples were subjected to SDS-PAGE. ab108504 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-GNB2 antibody [EP3262Y] (ab108504)](https://www.abcam.com/ps/products/108/ab108504/Images/ab108504-121201-anti-gnb2-antibody-ep3262y-western-blot.gif)
Western blot - Anti-GNB2 antibody [EP3262Y] (ab108504)All lanes : Anti-GNB2 antibody [EP3262Y] (ab108504) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
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![Anti-GNB2 antibody [EP3262Y] (ab108504)](https://www.abcam.com/ps/products/108/ab108504/Images/ab108504-3-benefits-of-recombinant-antibodies.png)
Anti-GNB2 antibody [EP3262Y] (ab108504)
