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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I

Anti-TPN antibody [7F6] (ab252869)

Price and availability

321 638 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-TPN antibody [7F6] (ab252869)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [7F6] to TPN
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-TPN antibody [7F6]
    See all TPN primary antibodies
  • Description

    Rat monoclonal [7F6] to TPN
  • Host species

    Rat
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, HUVEC, Jurkat, Daudi, Raji and HeLa whole lysates.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    7F6
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • MHC
    • Class I
    • Signal Transduction
    • Protein Trafficking
    • ER Proteins
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins

Images

  • Western blot - Anti-TPN antibody [7F6] (ab252869)
    Western blot - Anti-TPN antibody [7F6] (ab252869)
    All lanes : Anti-TPN antibody [7F6] (ab252869) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 2 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 4 : Daudi (human Burkitts lymphoma lymphoblast), whole cell lysate
    Lane 5 : Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 48 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The double bands observed in lane 5 is consistent with what has been described in the literatures (PMID: 29107940, 17620360). 

    Exposure time: lane 1-3: 6 seconds; lane 4-5: 3 seconds.

  • Western blot - Anti-TPN antibody [7F6] (ab252869)
    Western blot - Anti-TPN antibody [7F6] (ab252869)
    All lanes : Anti-TPN antibody [7F6] (ab252869) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HeLa treated with 100 U/ml IFN gamma for 24 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 48 kDa
    Observed band size: 47 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Anti-TPN antibody [7F6] (ab252869)
    Anti-TPN antibody [7F6] (ab252869)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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