Anti-Talin 1 and 2 antibody [8D4] (ab11188)
Key features and details
- Mouse monoclonal [8D4] to Talin 1 and 2
- Suitable for: WB, ICC
- Reacts with: Chicken, Chinese hamster
- Isotype: IgG1
Overview
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Product name
Anti-Talin 1 and 2 antibody [8D4]
See all Talin 1 and 2 primary antibodies -
Description
Mouse monoclonal [8D4] to Talin 1 and 2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Chicken, Chinese hamster
Does not react with: Fish -
Immunogen
Full length native protein (purified) corresponding to Chicken Talin 1 and 2.
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Positive control
- WB: CHO cells ICC: Chicken UMNSAH/DF1 cells
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General notes
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR228677. Lot numbers higher than GR228677 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
For continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Proprietary Purification -
Purification notes
Purified from Tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
8D4 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry analysis of Chicken UMNSAH/DF1 cells labeling Talin 1 and 2 with ab11188 at 5 μg/mL. Cells were fixed and permeabilized with methanol followed by aceton. The antibody was developed using Goat Anti-Mouse IgG Atto-488 conjugate.
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Lane 1 : Anti-Talin 1 and 2 antibody [8D4] (ab11188) at 2 µg/ml
Lane 2 : Anti-Talin 1 and 2 antibody [8D4] (ab11188) at 1 mg/ml
All lanes : Whole extract of CHO cells
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase
Predicted band size: 270 kDachemiluminescent substrate.