Anti-CBX1 / HP1 beta antibody [MAC353] (ab10811)
![Anti-CBX1 / HP1 beta antibody [MAC353] (ab10811)](https://www.abcam.com/ps/products/10/ab10811/Images/ab10811-269777-anti-cbx1-hp1-beta-antibody-mac353-chip-grade-western-blot.jpg "Anti-CBX1 / HP1 beta antibody [MAC353] (ab10811)")
Key features and details
- Rat monoclonal [MAC353] to CBX1 / HP1 beta
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-CBX1 / HP1 beta antibody [MAC353]
See all CBX1 / HP1 beta primary antibodies -
Description
Rat monoclonal [MAC353] to CBX1 / HP1 beta -
Host species
Rat -
Specificity
Ab10811 recognises the M31 molecule in mouse and the homologous HP1 HS beta molecule in man. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Fusion protein corresponding to Human CBX1/ HP1 beta (C terminal).
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Positive control
- Murine and human nuclear extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 5% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
MAC353 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-CBX1 / HP1 beta antibody [MAC353] - ChIP Grade (ab10811)
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: CBX1 knockout HAP1 cell lysate (40 µg)
Lane 3: MCF7 cell lysate (40 µg)
Lane 4: A431 cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab10811 observed at 26 kDa. Red - loading control, ab181602, observed at 37 kDa.ab10811 was shown to specifically react with CBX1 / HP1 beta when CBX1 / HP1 beta knockout samples were used. Wild-type and CBX1 / HP1 beta knockout samples were subjected to SDS-PAGE. Ab10811 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) (ab253031) preadsorbed and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-CBX1 / HP1 beta antibody [MAC353] - ChIP Grade (ab10811)](https://www.abcam.com/ps/products/10/ab10811/Images/ab10811-18470-anti-cbx1-hp1-beta-antibody-mac353-chip-grade-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CBX1 / HP1 beta antibody [MAC353] - ChIP Grade (ab10811) This image is courtesy of Scott Slattery and Mike ManciniHeLA cells were stained with ab10811 in panel one. In panel two they were stained with ab10811 (green), DAPI (blue) and SH-CREST (red), which stains the centromeres. Fix 30 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with 1 mg/ml Na borohydride or 100 mM ammonium chloride in PEM. Permeablize 30 minutes with 0.5% TX-100 in PEM. Block 30 minutes in 5% milk in TBST. Primary antibody incubated overnight at 4oC diluted 1/100 in 5% milk in TBST. Secondary antibody incubated 1 hour at RT diluted in 5% milk in TBST. Post-fix 20 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with ammonium chloride in PEM. Counterstain with DAPI in TBST. Mount with ProLong Gold antifade reagent from Invitrogen. Notes: Ample washing between each step. TBST = Tris buffered saline + 0.1% Tween. PEM = 80 mM K-PIPES, pH 6.8, 5 mM EGTA, 2 mM MgCl2. -
![Immunocytochemistry/ Immunofluorescence - Anti-CBX1 / HP1 beta antibody [MAC353] - ChIP Grade (ab10811)](https://www.abcam.com/ps/products/10/ab10811/Images/ab10811-18469-anti-cbx1-hp1-beta-antibody-mac353-chip-grade-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CBX1 / HP1 beta antibody [MAC353] - ChIP Grade (ab10811) Image from Talasz H et al., J Biol Chem. 2005 Nov 18;280(46):38814-22. Epub 2005 Sep 15. Fig 1.; doi: 10.1074/jbc.M505563200; November 18, 2005 The Journal of Biological Chemistry, 280, 38814-38822.Immunofluorescence analysis of mouse erythroleukemia cell nuclei, staining CBX1 / HP1 beta in heterochromatin, with ab10811.
Cells were fixed with paraformaldehyde, permeabilized using Triton X-100 and blocked for 30 min with 2.5% BSA. Cells were incubated with primary antibody (1/50) before incubating with a Cy3-conjugated goat anti-rat IgG to detect staining.
