Anti-CDK1 (phospho Y15) antibody (ab254121)
Key features and details
- Rabbit polyclonal to CDK1 (phospho Y15)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CDK1 (phospho Y15) antibody
See all CDK1 primary antibodies -
Description
Rabbit polyclonal to CDK1 (phospho Y15) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Rat CDK1 (phospho Y15). The exact sequence is proprietary.
Database link: P39951 -
Positive control
- WB: T-47D cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: HEPES, 0.87% Sodium chloride, 50% Glycerol, 0.01% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CDK1 (phospho Y15) antibody (ab254121)All lanes : Anti-CDK1 (phospho Y15) antibody (ab254121) at 1/1000 dilution
Lane 1 : T-47D (human ductal breast epithelial tumor cell line) cell lysate
Lane 2 : T-47D (treated with EGF, 30 ng/ml for 30 minutes) cell lysate
Lane 3 : T-47D cell lysate - treated with Lambda phosphatase (1200 units for 30 minutes)
Lane 4 : T-47D (treated with EGF, 30 ng/ml for 30 minutes) cell lysate - treated with Lambda phosphatase (1200 units for 30 minutes)
Predicted band size: 34 kDa