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Cancer Cell cycle Kinases/phosphatases Cdks

Anti-CDK5RAP3 antibody (ab70776)

Anti-CDK5RAP3 antibody (ab70776)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to CDK5RAP3
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CDK5RAP3 antibody
    See all CDK5RAP3 primary antibodies
  • Description

    Rabbit polyclonal to CDK5RAP3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to a region between residue 450 and the C-terminus (residue 506) of human CDK5RAP3 (NP_788276.1)

  • Positive control

    • HeLa and 293T whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab70776 was affinity purified using an epitope specific to CDK5RAP3 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Cdks
    • Cell Biology
    • Apoptosis
    • Intracellular
    • NFkB
    • RelA (p65)
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Cancer
    • Tumor biomarkers
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • NFkB
    • RelA (p65)

Images

  • Western blot - Anti-CDK5RAP3 antibody (ab70776)
    Western blot - Anti-CDK5RAP3 antibody (ab70776)
    All lanes : Anti-CDK5RAP3 antibody (ab70776) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 57 kDa
    Observed band size: 57 kDa
    Additional bands at: 220 kDa, 28 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-CDK5RAP3 antibody (ab70776)
    Immunoprecipitation - Anti-CDK5RAP3 antibody (ab70776)
    Detection of CDK5RAP3 by Western Blot of Immunprecipitate. ab70776 at 1µg/ml staining CDK5RAP3 in HeLa whole cell lysate immunoprecipitated using ab70776 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Chemiluminescence with exposure time of 30 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: Flow Cyt, IHC-P, IP, WB

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