Recombinant human CDK6 + CCND3 protein (Tagged) (ab271462)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, His tag N-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant human CDK6 + CCND3 protein (Tagged)
See all CDK6 + CCND3 proteins and peptides -
Biological activity
Specific activity: 12 pmol/min/µg
Assay was done in a Kinase buffer using Histone H1 (0.1 mg/ml) as a substrate with 20 M ATP at 30°C for 60 min.
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Purity
>= 80 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
MEKDGLCRADQQYECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTG EEGMPLSTIREVAVLRHLETFEHPNVVRLFDVCTVSRTDRETKLTLVFEH VDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHRVVHRDLKPQN ILVTSSGQIKLADFGLARIYSFQMALTSVVVTLWYRAPEVLLQSSYATPV DLWSVGCIFAEMFRRKPLFRGSSDVDQLGKILDVIGLPGEEDWPRDVALP RQAFHSKSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYSALSHPYF QDLERCKENLDSHLPPSQNTSELNTA -
Predicted molecular weight
71 kDa -
Amino acids
1 to 326 -
Tags
His tag N-Terminus
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Amino Acid Sequence 2
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Species
Human -
Sequence
MELLCCEGTRHAPRAGPDPRLLGDQRVLQSLLRLEERYVPRASYFQCVQR EIKPHMRKMLAYWMLEVCEEQRCEEEVFPLAMNYLDRYLSCVPTRKAQLQ LLGAVCMLLASKLRETTPLTIEKLCIYTDHAVSPRQLRDWEVLVLGKLKW DLAAVIAHDFLAFILHRLSLPRDRQALVKKHAQTFLALCATDYTFAMYPP SMIATGSIGAAVQGLGACSMSGDELTELLAGITGTEVDCLRACQEQIEAA LRESLREASQTSSSPAPKAPRGSSSQGPSQTSTPTDVTAIHL -
Amino acids
1 to 292 -
Tags
His tag N-Terminus
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Preparation and Storage
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Alternative names
- CCND 3
- CCND3
- CDK 6
see all -
Relevance
CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of Cdk6 first appears in mid-G1 phase, and is controlled by regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Cyclin D3 is a G1 cyclin closely related to cyclin D1 and D2. Cyclins D1, D2, and D3 are members of a growing superfamily of cyclins, proteins that govern transitions through distinct phases of the cell cycle by regulating the activity of cyclin-dependent kinases (cdks). All three known members of the D-type subfamily form active kinase complexes in vivo with a distinct subset of cdks, particularly with cdk4 and cdk6. -
Cellular localization
CCND3: Cytoplasm. Nucleus
Images
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SDS-PAGE - Recombinant Human CDK6 + CCND3 protein (Tagged) (ab271462)SDS-PAGE analysis of ab271462.
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Functional Studies - Recombinant Human CDK6 + CCND3 protein (Tagged) (ab271462)Specific activity of ab271462.