Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Adherent cells, Plasma, Serum, Suspension cells, Tissue, Tissue Homogenate
- Sensitivity: 1 mU/well
Overview
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Product name
Creatine Kinase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate -
Assay type
Enzyme activity -
Sensitivity
Range
2 nmol/well - 10 nmol/wellAssay time
1h 0mSpecies reactivity
Reacts with: Mammals, Other speciesProduct overview
Creatine Kinase Activity Assay Kit (Colorimetric) ab155901 uses a high-throughput adaptable, simple and sensitive assay for creatine kinase (CK) activity.
In the creatine kinase assay protocol, creatine kinase (CK) converts creatine into phosphocreatine and ADP. The phosphocreatine and ADP then react with the CK enzyme mix to form an intermediate, which reduces a colorless probe to a colored product with strong absorbance at λ= 450 nm.
This assay kit can detect CK activity to less than 1 mU.
Creatine kinase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze every 1-2 min for 10-40 min with microplate reader in kinetic mode at 37ºCNotes
Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme (EC 2.7.3.2). It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.Platform
Microplate readerProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests ATP Orange 1 vial CK Assay Buffer WM 1 x 25ml CK Developer Red 1 vial CK Enzyme Mix Green 1 vial CK Positive Control Purple 1 vial CK Substrate Blue 1 x 1ml NADH Standard Yellow 1 vial -
Research areas
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests ATP Orange 1 vial CK Assay Buffer WM 1 x 25ml CK Developer Red 1 vial CK Enzyme Mix Green 1 vial CK Positive Control Purple 1 vial CK Substrate Blue 1 x 1ml NADH Standard Yellow 1 vial -
Research areas
Images
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Functional studies - Creatine Kinase Activity Assay Kit (ab155901)
Creatine Kinase Activity measured in various samples.
Initial protein concentration for tissue lysates varied from 1 mg/mL to 3 mg/mL. 40 μl of filtered tissue lysates were used. 30 μl of filtered biological fluids were used.
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Functional Studies - Creatine Kinase Activity Assay Kit (Colorimetric) (ab155901) Sengle, Gerhard et al., PLoS genetics?vol. 11,6 e1005340., Table 1, doi:10.1371/journal.pgen.1005340Creatine kinase activities in blood samples from wildtype and Fbn2 null mice.
Mean +/- standard deviation for a given genotype and age is shown, except where only one animal was tested. N = number of animals tested. Units are nmol NADH/min/ml. -
Functional studies - Creatine Kinase Activity Assay KitTypical NADH standard calibration curve using colorimetric reading.
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Figure 1Creatine kinase activity tested in 5 µl human serum and 192 ng rat heart lysate. Assay performed following kit protocol.