Human Contactin 2 ELISA Kit (ab267632)
Key features and details
- Sensitivity: 0.12 ng/ml
- Range: 0.102 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Contactin 2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.12 ng/ml -
Range
0.102 ng/ml - 25 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 141.9 137% - 147% Serum 117.9 99% - 135% Plasma 120.8 84% - 138% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Contactin 2 ELISA Kit is designed for the quantitative determination of Contactin 2 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human Contactin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Contactin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Contactin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Contactin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ALK-1, B7-H2, BLAME, BMP-8, CD28, Common beta Chain, Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF17, FOLR2, Galectin-8, Glypican 5, GHR, IFN-gamma R1, IL-22 R alpha 1, IL-22BP, IL-23 R, IL-31 RA, IL-7 R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo,Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Semaphorin 7A, Siglec-11.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Anti-Human Contactin 2 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Human Contactin 2 Detection Antibody 2 vials Human Contactin 2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
May play a role in the initial growth and guidance of axons. May be involved in cell adhesion. -
Sequence similarities
Belongs to the immunoglobulin superfamily. Contactin family.
Contains 4 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Cell membrane. Attached to the neuronal membrane by a GPI-anchor and is also released from neurons. - Information by UniProt
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Alternative names
- Axonal glycoprotein TAG 1
- Axonal glycoprotein TAG-1
- Axonal glycoprotein TAG1
see all -
Database links
- Entrez Gene: 6900 Human
- Omim: 190197 Human
- SwissProt: Q02246 Human
- Unigene: 519220 Human
Images
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Example standard curve
These standard curves are for demonstration only. A standard curve must be run with each assay.