Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SIRP alpha antibody [EPR16264] (ab191419)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16264] to SIRP alpha
  • Suitable for: ICC/IF, WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SIRP alpha antibody [EPR16264]
    See all SIRP alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR16264] to SIRP alpha

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Rat
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP1, SW480, C6, PC12, NIH 3T3 and Human fetal brain lysates. Flow Cyt: THP1 cells. ICC/IF: C6 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SIRP alpha antibody [EPR16264] (ab191419)
    Western blot - Anti-SIRP alpha antibody [EPR16264] (ab191419)
    All lanes : Anti-SIRP alpha antibody [EPR16264] (ab191419) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : SIRPA knockout HAP1 whole cell lysate
    Lane 3 : THP1 whole cell lysate
    Lane 4 : Jurkat whole cell lysate (negative control)

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab191419 observed at 55 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab191419 was shown to recognize SIRP alpha in wild-type HAP1 cells as signal was lost at the expected MW in SIRPA knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SIRPA knockout samples were subjected to SDS-PAGE. Ab191419 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody [EPR16264] (ab191419)
    Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody [EPR16264] (ab191419)
    Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) labeling SIRP alpha with ab191419 at 1/500. ab150077 Alexa Fluor® 488 Goat anti-Rabbit at 1/1000 was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI was used to stain nuclei blue.
     
    Confocal image showing membranous staining on C6 cell line.
  • Flow Cytometry - Anti-SIRP alpha antibody [EPR16264] (ab191419)
    Flow Cytometry - Anti-SIRP alpha antibody [EPR16264] (ab191419)

    Flow Cytometric analysis of THP1 cells (2% paraformaldehyde-fixed)  labeling SIRP alpha with ab191419 at 1/50 dilution (red) or a Rabbit monoclonal IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution

  • Western blot - Anti-SIRP alpha antibody [EPR16264] (ab191419)
    Western blot - Anti-SIRP alpha antibody [EPR16264] (ab191419)
    All lanes : Anti-SIRP alpha antibody [EPR16264] (ab191419) at 1/5000 dilution

    Lane 1 : THP1 cell lysate
    Lane 2 : SW480 cell lysate
    Lane 3 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa

  • Western blot - Anti-SIRP alpha antibody [EPR16264] (ab191419)
    Western blot - Anti-SIRP alpha antibody [EPR16264] (ab191419)
    All lanes : Anti-SIRP alpha antibody [EPR16264] (ab191419) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa

  • Anti-SIRP alpha antibody [EPR16264] (ab191419)
    Anti-SIRP alpha antibody [EPR16264] (ab191419)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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