Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free (ab271957)
![Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free (ab271957)](https://www.abcam.com/ps/products/271/ab271957/Images/ab271957-2-ab191419343547antiSIRPalphaantibodyEPR16264immunocytochemistryimmunofluorescencec6rat.jpg "Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free (ab271957)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16264] to SIRP alpha - BSA and Azide free
- Suitable for: WB, Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free
See all SIRP alpha primary antibodies -
Description
Rabbit monoclonal [EPR16264] to SIRP alpha - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: THP1 cells. ICC/IF: C6 cells
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General notes
ab271957 is the carrier-free version of ab191419. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16264 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free (ab271957)Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) labeling SIRP alpha with ab191419 at 1/500. ab150077 Alexa Fluor® 488 Goat anti-Rabbit at 1/1000 was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI was used to stain nuclei blue.Confocal image showing membranous staining on C6 cell line.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191419).
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![Flow Cytometry - Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free (ab271957)](https://www.abcam.com/ps/products/271/ab271957/Images/ab271957-1-ab191419230818antisirpalphaantibodyepr16264flowcytometry.jpg)
Flow Cytometry - Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free (ab271957)Flow Cytometric analysis of THP1 cells (2% paraformaldehyde-fixed) labeling SIRP alpha with ab191419 at 1/50 dilution (red) or a Rabbit monoclonal IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191419). -
![Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free (ab271957)](https://www.abcam.com/ps/products/271/ab271957/Images/ab271957-3-benefits-of-recombinant-antibodies.png)
Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free (ab271957)
