Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free (ab271957)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16264] to SIRP alpha - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free
    See all SIRP alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR16264] to SIRP alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: THP1 cells. ICC/IF: C6 cells

  • General notes

    ab271957 is the carrier-free version of ab191419. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free (ab271957)
    Immunocytochemistry/ Immunofluorescence - Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free (ab271957)
    Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) labeling SIRP alpha with ab191419 at 1/500. ab150077 Alexa Fluor® 488 Goat anti-Rabbit at 1/1000 was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI was used to stain nuclei blue.
     
    Confocal image showing membranous staining on C6 cell line.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191419).
  • Flow Cytometry - Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free (ab271957)
    Flow Cytometry - Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free (ab271957)

    Flow Cytometric analysis of THP1 cells (2% paraformaldehyde-fixed)  labeling SIRP alpha with ab191419 at 1/50 dilution (red) or a Rabbit monoclonal IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191419).
  • Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free (ab271957)
    Anti-SIRP alpha antibody [EPR16264] - BSA and Azide free (ab271957)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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