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Signal Transduction Metabolism Energy Metabolism

Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)

Price and availability

321 638 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP737Y] to ATP citrate lyase (phospho T447 + S451)
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y]
    See all ATP citrate lyase primary antibodies
  • Description

    Rabbit monoclonal [EP737Y] to ATP citrate lyase (phospho T447 + S451)
  • Host species

    Rabbit
  • Specificity

    ab53007 only detects ATP-citrate lyase phosphorylated on Threonine 447 and Serine 451.
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    corresponding to Human ATP citrate lyase.

  • Positive control

    • WB: Untreated and insulin treated NIH/3T3, NIH/3T3, HeLa and C6 whole cell lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP737Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)
    Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)
    All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100nM Calyculin A for 30 minutes whole cell lysates
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Calyculin A for 15 minutes whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 122 kDa
    Observed band size: 122 kDa



    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)
    Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)
    All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution (Purified)

    Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lane 2 : C6 (Rat glial tumor glial cell) treated with 100ng Calyculin A for 60 minutes whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 122 kDa
    Observed band size: 122 kDa



    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)
    Western blot - Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)
    All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution (unpurified)

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) treated with insulin whole cell lysates.
    Lane 2 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysates.

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated. at 1/2000 dilution

    Predicted band size: 122 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST

  • Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)
    Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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