Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP737Y] to ATP citrate lyase (phospho T447 + S451)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y]
See all ATP citrate lyase primary antibodies -
Description
Rabbit monoclonal [EP737Y] to ATP citrate lyase (phospho T447 + S451) -
Host species
Rabbit -
Specificity
ab53007 only detects ATP-citrate lyase phosphorylated on Threonine 447 and Serine 451. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman -
Immunogen
corresponding to Human ATP citrate lyase.
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Positive control
- WB: Untreated and insulin treated NIH/3T3, NIH/3T3, HeLa and C6 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP737Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100nM Calyculin A for 30 minutes whole cell lysates
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Calyculin A for 15 minutes whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 122 kDa
Observed band size: 122 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution (Purified)
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 2 : C6 (Rat glial tumor glial cell) treated with 100ng Calyculin A for 60 minutes whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 122 kDa
Observed band size: 122 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-ATP citrate lyase (phospho T447 + S451) antibody [EP737Y] (ab53007) at 1/1000 dilution (unpurified)
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) treated with insulin whole cell lysates.
Lane 2 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysates.
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated. at 1/2000 dilution
Predicted band size: 122 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
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