Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCTAIRE1 antibody [EPR13210] - BSA and Azide free (ab250427)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13210] to PCTAIRE1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PCTAIRE1 antibody [EPR13210] - BSA and Azide free
    See all PCTAIRE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13210] to PCTAIRE1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250427 is the carrier-free version of ab181208.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PCTAIRE1 antibody [EPR13210] - BSA and Azide free (ab250427)
    Western blot - Anti-PCTAIRE1 antibody [EPR13210] - BSA and Azide free (ab250427)

    This data was developed using ab181208, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: PCTAIRE1 knockout HAP1 cell lysate (20 µg)

    Lane 3: HEK293 cell lysate (20 µg)

    Lane 4: HepG2 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab181208 observed at 63 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab181208 was shown to recognize PCTAIRE1 when PCTAIRE1 knockout samples were used, along with additional cross-reactive bands. Wild-type and PCTAIRE1 knockout samples were subjected to SDS-PAGE. ab181208 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-PCTAIRE1 antibody [EPR13210] - BSA and Azide free (ab250427)
    Flow Cytometry - Anti-PCTAIRE1 antibody [EPR13210] - BSA and Azide free (ab250427)
    This data was developed using ab181208, the same antibody clone in a different buffer formulation.Flow cytometric analysis of BxPC-3 cells (2% paraformaldehyde-fixed) labeling PCTAIRE1 with ab181208 at 1/20 dilution (red) or a rabbit IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as a secondary antibody.
  • Western blot - Anti-PCTAIRE1 antibody [EPR13210] - BSA and Azide free (ab250427)
    Western blot - Anti-PCTAIRE1 antibody [EPR13210] - BSA and Azide free (ab250427)
    All lanes : Anti-PCTAIRE1 antibody [EPR13210] (ab181208) at 1/1000 dilution

    Lane 1 : Human testis cell lysate
    Lane 2 : Human fetal heart cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 56 kDa



    This data was developed using ab181208, the same antibody clone in a different buffer formulation.

    These two bands may be the longer form of PCTAIRE1 and the alternatively spliced form.

  • Western blot - Anti-PCTAIRE1 antibody [EPR13210] - BSA and Azide free (ab250427)
    Western blot - Anti-PCTAIRE1 antibody [EPR13210] - BSA and Azide free (ab250427)
    Anti-PCTAIRE1 antibody [EPR13210] (ab181208) at 1/1000 dilution + BxPC-3 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 56 kDa



    This data was developed using ab181208, the same antibody clone in a different buffer formulation.

    These two bands may be the longer form of PCTAIRE1 and the alternatively spliced form.

  • Anti-PCTAIRE1 antibody [EPR13210] - BSA and Azide free (ab250427)
    Anti-PCTAIRE1 antibody [EPR13210] - BSA and Azide free (ab250427)

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