Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATP citrate lyase antibody (ab133403)

Key features and details

  • Rabbit polyclonal to ATP citrate lyase
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-ATP citrate lyase antibody
    See all ATP citrate lyase primary antibodies

  • Description

    Rabbit polyclonal to ATP citrate lyase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human ATP citrate lyase aa 1050 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab133779)

  • Positive control

    • WB: Human Brain and Human Testis tissue lysates, HAP1, HepG2; HEK293; PC3.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rat
Chicken
Cow
Zebrafish
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).

Target

  • Function

    ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.

  • Sequence similarities

    In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
    In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
    Contains 1 ATP-grasp domain.

  • Post-translational
    modifications

    ISGylated.
    Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
    Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ACL antibody
    • Acly antibody
    • ACLY_HUMAN antibody
    • ATP citrate (pro-S) lyase antibody
    • ATP citrate lyase antibody
    • ATP citrate synthase antibody
    • ATP-citrate (pro-S-)-lyase antibody
    • ATP-citrate synthase antibody
    • ATPcitrate synthase antibody
    • ATPCL antibody
    • Citrate cleavage enzyme antibody
    • CLATP antibody
    • OTTHUMP00000164773 antibody
    see all

Images

  • Western blot - Anti-ATP citrate lyase antibody (ab133403)
    Western blot - Anti-ATP citrate lyase antibody (ab133403)
    All lanes : Anti-ATP citrate lyase antibody (ab133403) at 1 µg/ml

    Lane 1 : Wild-type HepG2 whole cell lysate
    Lane 2 : Hap1 knockout HepG2 whole cell lysate
    Lane 3 : ACLY whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 121 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab133403 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab133403 was shown to specifically react with in wild-type HepG2 cells as signal was lost in Hap1 knockout cells. Wild-type and Hap1 knockout samples were subjected to SDS-PAGE. Ab133403 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ATP citrate lyase antibody (ab133403)
    Western blot - Anti-ATP citrate lyase antibody (ab133403)
    All lanes : Anti-ATP citrate lyase antibody (ab133403) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 121 kDa
    Observed band size: 121 kDa
    Additional bands at: 19 kDa, 39 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes

References (0)

Publishing research using ab133403? Please let us know so that we can cite the reference in this datasheet.

ab133403 has not yet been referenced specifically in any publications.

Images

  • Western blot - Anti-ATP citrate lyase antibody (ab133403)
    Western blot - Anti-ATP citrate lyase antibody (ab133403)
    All lanes : Anti-ATP citrate lyase antibody (ab133403) at 1 µg/ml

    Lane 1 : Wild-type HepG2 whole cell lysate
    Lane 2 : Hap1 knockout HepG2 whole cell lysate
    Lane 3 : ACLY whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 121 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab133403 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab133403 was shown to specifically react with in wild-type HepG2 cells as signal was lost in Hap1 knockout cells. Wild-type and Hap1 knockout samples were subjected to SDS-PAGE. Ab133403 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ATP citrate lyase antibody (ab133403)
    Western blot - Anti-ATP citrate lyase antibody (ab133403)
    All lanes : Anti-ATP citrate lyase antibody (ab133403) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 121 kDa
    Observed band size: 121 kDa
    Additional bands at: 19 kDa, 39 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes

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