Anti-ATP citrate lyase antibody (ab133403)
Key features and details
- Rabbit polyclonal to ATP citrate lyase
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATP citrate lyase antibody
See all ATP citrate lyase primary antibodies -
Description
Rabbit polyclonal to ATP citrate lyase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human ATP citrate lyase aa 1050 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab133779) -
Positive control
- WB: Human Brain and Human Testis tissue lysates, HAP1, HepG2; HEK293; PC3.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatChickenCowZebrafishApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).Target
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Function
ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. -
Sequence similarities
In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
Contains 1 ATP-grasp domain. -
Post-translational
modificationsISGylated.
Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 395373 Chicken
- Entrez Gene: 47 Human
- Entrez Gene: 104112 Mouse
- Entrez Gene: 24159 Rat
- Omim: 108728 Human
- SwissProt: Q32PF2 Cow
- SwissProt: P53396 Human
- SwissProt: Q91V92 Mouse
see all -
Alternative names
- ACL antibody
- Acly antibody
- ACLY_HUMAN antibody
see all
Images
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Western blot - Anti-ATP citrate lyase antibody (ab133403)All lanes : Anti-ATP citrate lyase antibody (ab133403) at 1 µg/ml
Lane 1 : Wild-type HepG2 whole cell lysate
Lane 2 : Hap1 knockout HepG2 whole cell lysate
Lane 3 : ACLY whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 121 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab133403 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab133403 was shown to specifically react with in wild-type HepG2 cells as signal was lost in Hap1 knockout cells. Wild-type and Hap1 knockout samples were subjected to SDS-PAGE. Ab133403 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-ATP citrate lyase antibody (ab133403)All lanes : Anti-ATP citrate lyase antibody (ab133403) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lane 3 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 121 kDa
Observed band size: 121 kDa
Additional bands at: 19 kDa, 39 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab133403 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-ATP citrate lyase antibody (ab133403)All lanes : Anti-ATP citrate lyase antibody (ab133403) at 1 µg/ml
Lane 1 : Wild-type HepG2 whole cell lysate
Lane 2 : Hap1 knockout HepG2 whole cell lysate
Lane 3 : ACLY whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 121 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab133403 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab133403 was shown to specifically react with in wild-type HepG2 cells as signal was lost in Hap1 knockout cells. Wild-type and Hap1 knockout samples were subjected to SDS-PAGE. Ab133403 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-ATP citrate lyase antibody (ab133403)All lanes : Anti-ATP citrate lyase antibody (ab133403) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lane 3 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 121 kDa
Observed band size: 121 kDa
Additional bands at: 19 kDa, 39 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
