Anti-DLX3 antibody [EPR8762(2)] (ab178428)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8762(2)] to DLX3
- Suitable for: IP, WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-DLX3 antibody [EPR8762(2)]
See all DLX3 primary antibodies -
Description
Rabbit monoclonal [EPR8762(2)] to DLX3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DLX3 aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O60479 -
Positive control
- JAR and 293T cell line lysates; Human adult and fetal skin tissue lysates; Rat skin tissue lysate.
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General notes
This antibody was developed through collaboration with the lab of Maria Morasso at the National Cancer Institute, NIH.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8762(2) -
Isotype
IgG -
Research areas
Images
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Western blot analysis on immunoprecipitation pellet from (1) Human fetal skin lysate or (2) 1XPBS (negative control) immunoprecipitated using ab178428 with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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All lanes : Anti-DLX3 antibody [EPR8762(2)] (ab178428) at 1/1000 dilution
Lane 1 : JAR cell line lysate
Lane 2 : Human skin tissue lysate
Lane 3 : Rat skin tissue lysate
Lane 4 : 293T cell line lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody
Developed using the ECL technique.
Predicted band size: 32 kDa
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