Antibodies, Proteins, Kits and Reagents for Life Science

300 865 ₸ Product size

100 µl 300 865 ₸

Order now and get it on Tuesday March 02, 2021

Anti-RBPJK antibody [EPR13479] (ab180588)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13479] to RBPJK
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-RBPJK antibody [EPR13479]
See all RBPJK primary antibodies
Description

Rabbit monoclonal [EPR13479] to RBPJK
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: 293 cells, MCF7, Raji, F9, C9, and HeLa cell line lysates; ICC/IF: 293T cells; Flow Cyt: HEK-293;
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13479
Isotype

IgG
Research areas

Western blot - Anti-RBPJK antibody [EPR13479] (ab180588)

All lanes : Anti-RBPJK antibody [EPR13479] (ab180588) at 1/1000 dilution (Purified)

Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 15 µg
Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 3 : F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysates at 15 µg
Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 56 kDa
Observed band size: 56-60 kDa
why is the actual band size different from the predicted?

The double bands are consistent with what has been described in the literature PMID: 9102301 and 24603501
Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] (ab180588)

Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling RBPJK with Purified ab180588 at 1:100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-RBPJK antibody [EPR13479] (ab180588)

Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling RBPJK with Purified ab180588 at 1:100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-RBPJK antibody [EPR13479] (ab180588)

All lanes : Anti-RBPJK antibody [EPR13479] (ab180588) at 0.4 µg/ml (Unpurified)

Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : Raw264.7 (Mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 56 kDa

Blocking and diluting buffer: 5% NFDM/TBST.

Exposure time: Lane 1-3: 3 minutes
Lane 4: 45 seconds
Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] (ab180588)

Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling RBPJK with unpurified ab180588 at 1/100 (green). Dapi shown in blue.
Western blot - Anti-RBPJK antibody [EPR13479] (ab180588)

All lanes : Anti-RBPJK antibody [EPR13479] (ab180588) at 1/1000 dilution (Unpurified)

Lane 1 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 20ug prepared using RIPA lysis method
Lane 2 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 20ug prepared using 1% SDS hot lysis method

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Diluting buffer and concentration 5% NFDM/TBST.

Extra band at: 90kDa
We are unsure as to the identity of this extra band.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
Western blot - Anti-RBPJK antibody [EPR13479] (ab180588)

All lanes : Anti-RBPJK antibody [EPR13479] (ab180588) at 1/2000 dilution (Unpurified)

Lane 1 : 293 cell line lysate
Lane 2 : MCF7 cell line lysate
Lane 3 : HeLa cell line lysate

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Anti-RBPJK antibody [EPR13479] (ab180588)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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