Anti-RBPJK antibody [7A11] - BSA and Azide free (ab255852)
![Anti-RBPJK antibody [7A11] - BSA and Azide free (ab255852)](https://www.abcam.com/ps/products/255/ab255852/Images/ab255852-387092-AntiRBPJK-antibody-7a11-wb-various-cells.jpg "Anti-RBPJK antibody [7A11] - BSA and Azide free (ab255852)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [7A11] to RBPJK - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-RBPJK antibody [7A11] - BSA and Azide free
See all RBPJK primary antibodies -
Description
Rabbit monoclonal [7A11] to RBPJK - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Fusion protein corresponding to Human RBPJK. Human CBF1 was expressed in Escherichia coli BL21(DE3)RIL as a His/glutathione S-transferase (GST) fusion protein (VK91)
Database link: Q06330 -
Positive control
- WB: Daudi, Raji, MCF7, HeLa C6, RAW264.7, NIH/3T3 whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab255852 is the carrier-free version of ab252858. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
7A11 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-RBPJK antibody [7A11] - BSA and Azide free (ab255852)All lanes : Anti-RBPJK antibody [7A11] (ab252858) at 1/1000 dilution
Lane 1 : Daudi (human Burkitt’s lymphoma lymphoblast), whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 56 kDaBlocking buffer: 5% NFDM/TBST.
The molecular weight observed was in consistent with the literature (PMID:25512468) and the benchmark ab180588.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252858).
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![Western blot - Anti-RBPJK antibody [7A11] - BSA and Azide free (ab255852)](https://www.abcam.com/ps/products/255/ab255852/Images/ab255852-387091-AntiRBPJK-antibody-7a11-wb-various-mouse-and-rat--cells.jpg)
Western blot - Anti-RBPJK antibody [7A11] - BSA and Azide free (ab255852)All lanes : Anti-RBPJK antibody [7A11] (ab252858) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 56 kDaBlocking buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252858).
-
![Anti-RBPJK antibody [7A11] - BSA and Azide free (ab255852)](https://www.abcam.com/ps/products/255/ab255852/Images/ab255852-3-benefits-of-recombinant-antibodies.png)
Anti-RBPJK antibody [7A11] - BSA and Azide free (ab255852)
