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Epigenetics and Nuclear Signaling Transcription Other factors

Anti-RBPJK antibody [7A11] (ab252858)

Price and availability

307 566 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-RBPJK antibody [7A11] (ab252858)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [7A11] to RBPJK
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-RBPJK antibody [7A11]
    See all RBPJK primary antibodies
  • Description

    Rabbit monoclonal [7A11] to RBPJK
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Fusion protein. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Daudi, Raji, MCF7, HeLa C6, RAW264.7, NIH/3T3 whole cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    7A11
  • Isotype

    IgG2b
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Images

  • Western blot - Anti-RBPJK antibody [7A11] (ab252858)
    Western blot - Anti-RBPJK antibody [7A11] (ab252858)
    All lanes : Anti-RBPJK antibody [7A11] (ab252858) at 1/1000 dilution

    Lane 1 : Daudi (human Burkitt’s lymphoma lymphoblast), whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 56 kDa



    Blocking buffer: 5% NFDM/TBST.

    Exposur time: 

    lane 1, 2: 180 seconds
    lane 3, 4: 48 seconds

    The molecular weight observed was in consistent with the literature (PMID:25512468) and the benchmark ab180588.

  • Western blot - Anti-RBPJK antibody [7A11] (ab252858)
    Western blot - Anti-RBPJK antibody [7A11] (ab252858)
    All lanes : Anti-RBPJK antibody [7A11] (ab252858) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 56 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking buffer: 5% NFDM/TBST.

     

  • Anti-RBPJK antibody [7A11] (ab252858)
    Anti-RBPJK antibody [7A11] (ab252858)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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