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Epigenetics and Nuclear Signaling Transcription Other factors

Anti-CBF antibody (ab89034)

Anti-CBF antibody (ab89034)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse polyclonal to CBF
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CBF antibody
    See all CBF primary antibodies
  • Description

    Mouse polyclonal to CBF
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length human CBF protein (NP_005751.2).

  • Positive control

    • WB: NIH/3T3 cell lysate; K-562 cell lysate; CBF transfected 293T cell lysate ICC/IF: HeLa cell
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Images

  • Western blot - Anti-CBF antibody (ab89034)
    Western blot - Anti-CBF antibody (ab89034)
    Anti-CBF antibody (ab89034) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg

    Predicted band size: 121 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-CBF antibody (ab89034)
    Western blot - Anti-CBF antibody (ab89034)
    Anti-CBF antibody (ab89034) at 1 µg/ml + K-562 cell lysate at 50 µg

    Predicted band size: 121 kDa
    Observed band size: 131 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-CBF antibody (ab89034)
    Western blot - Anti-CBF antibody (ab89034)
    All lanes : Anti-CBF antibody (ab89034) at 1 µg/ml

    Lane 1 : CEBPZ transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 121 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-CBF antibody (ab89034)
    Immunocytochemistry/ Immunofluorescence - Anti-CBF antibody (ab89034)
    ab89034, at 10 µg/ml, staining CBF in Hela cell by Immunofluorescence.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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