Anti-CBF antibody (ab176579)
Key features and details
- Rabbit polyclonal to CBF
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CBF antibody
See all CBF primary antibodies -
Description
Rabbit polyclonal to CBF -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human CBF aa 1004-1054 (C terminal). The exact sequence is proprietary. NP_005751.2
Sequence:NKDNASL KQLRWEAERD DWLHNRDAKS IIKKKKHFKK KRIKTTQKTK KQRK
Database link: Q03701 -
Positive control
- HeLa and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176579 was affinity purified using an epitope specific to CBF immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CBF antibody (ab176579)All lanes : Anti-CBF antibody (ab176579) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg
Lane 2 : HeLa whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 5 µg
Lane 4 : 293T whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 121 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-CBF antibody (ab176579)Detection of CBF in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176579 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab176579 was used at 1 µg/ml. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
