Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free (ab247457)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free
    See all Synapsin I primary antibodies

  • Description

    Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247457 is the carrier-free version of ab76260. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247457 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free (ab247457)
    Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free (ab247457)
    All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/1000 dilution

    Lanes 1 & 3 : Human brain whole cell lysate - untreated
    Lanes 2 & 4 : Human brain whole cell lysate - treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 74 kDa
    Observed band size: 77 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76260, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer:

    Lanes 1 and 2: 5% NFDM/TBST.

    Lanes 3 and 4: 2% BSA/TBST.

    Exposure time:

    Lanes 1 and 2: 1 second.

    Lanes 3 and 4: 15 seconds.

  • Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free (ab247457)
    Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free (ab247457)
    All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/5000 dilution

    Lane 1 : Human brain whole cell lysate - untreated
    Lane 2 : Human brain whole cell lysate - treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 74 kDa
    Observed band size: 77 kDa why is the actual band size different from the predicted?



    This data was developed using ab76260, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free (ab247457)
    Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free (ab247457)
    All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/200 dilution

    Lane 1 : Mouse brain whole cell lysate - untreated
    Lane 2 : Mouse brain whole cell lysate - treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 74 kDa
    Observed band size: 77 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab76260, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free (ab247457)
    Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free (ab247457)
    All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/1000 dilution

    Lane 1 : Rat brain whole cell lysate - untreated
    Lane 2 : Rat brain whole cell lysate - treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 74 kDa
    Observed band size: 77 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab76260, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 2% BSA/TBST.

  • Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free (ab247457)
    Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free (ab247457)
    All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)

    Lane 1 : Human brain tissue lysate, untreated
    Lane 2 : Human brain tissue lysate, treated with AP

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 74 kDa
    Observed band size: 74 kDa



    This data was developed using ab76260, the same antibody clone in a different buffer formulation.

  • Dot Blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free (ab247457)
    Dot Blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free (ab247457)
    This data was developed using ab76260, the same antibody clone in a different buffer formulation.Dot blot analysis of Synapsin I (pS9) phospho peptide (lane 1) and Synapsin I non-phospho peptide (lane 2) labelling Synapsin I (pS9) with ab76260 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).Blocking and dilution buffer: 5% NFDM/TBST.Exposure time: 3 minutes.
  • Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free (ab247457)
    Anti-Synapsin I (phospho S9) antibody [EP2162Y] - BSA and Azide free (ab247457)

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