Anti-KIF1A antibody (ab91029)
Key features and details
- Rabbit polyclonal to KIF1A
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KIF1A antibody
See all KIF1A primary antibodies -
Description
Rabbit polyclonal to KIF1A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ELISAmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Dog
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Immunogen
Synthetic peptide corresponding to Human KIF1A aa 1500-1600 conjugated to keyhole limpet haemocyanin.
(Peptide available asab108241) -
Positive control
- This antibody gave a positive signal in both Mouse brain embryonic E14 and Mouse brain embryonic E18 tissue lysates. As well as human hippocampus formalin fixed paraffin embedded tissue section within immunohistochemistry.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.WB Use a concentration of 1 µg/ml. Detects a band of approximately 191 kDa (predicted molecular weight: 191 kDa).ELISA Use a concentration of 1 µg/ml.Notes IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 191 kDa (predicted molecular weight: 191 kDa).ELISA
Use a concentration of 1 µg/ml.Target
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Function
Motor for anterograde axonal transport of synaptic vesicle precursors. -
Sequence similarities
Belongs to the kinesin-like protein family. Unc-104 subfamily.
Contains 1 FHA domain.
Contains 1 kinesin-motor domain.
Contains 1 PH domain. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 547 Human
- Entrez Gene: 16560 Mouse
- Omim: 601255 Human
- SwissProt: Q12756 Human
- SwissProt: P33173 Mouse
- Unigene: 516802 Human
- Unigene: 276408 Mouse
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Alternative names
- ATSV antibody
- Axonal transporter of synaptic vesicles antibody
- C2orf20 antibody
see all
Images
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Western blot - Anti-KIF1A antibody (ab91029)All lanes : Anti-KIF1A antibody (ab91029) at 1 µg/ml
Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
Lane 2 : E18 Ms Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 191 kDa
Observed band size: 191 kDa
Additional bands at: 58 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF1A antibody (ab91029)IHC image of KIF1A staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91029, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
ELISA - Anti-KIF1A antibody (ab91029)ab91029 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 1% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab91029 has been referenced in 2 publications.
- Lannom MC et al. FMRP and MOV10 regulate Dicer1 expression and dendrite development. PLoS One 16:e0260005 (2021). PubMed: 34847178
- Hung CO & Coleman MP KIF1A mediates axonal transport of BACE1 and identification of independently moving cargoes in living SCG neurons. Traffic 17:1155-1167 (2016). WB ; Mouse . PubMed: 27484852
Images
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Western blot - Anti-KIF1A antibody (ab91029)All lanes : Anti-KIF1A antibody (ab91029) at 1 µg/ml
Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
Lane 2 : E18 Ms Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 191 kDa
Observed band size: 191 kDa
Additional bands at: 58 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF1A antibody (ab91029)IHC image of KIF1A staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91029, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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ELISA - Anti-KIF1A antibody (ab91029)
