Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸ 10 µl 117 264 ₸

Order now and get it on Tuesday March 02, 2021

Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11790(B)] to KIF1A
Suitable for: WB, ICC/IF, Flow Cyt, IP
Reacts with: Rat, Human

Product name

Anti-KIF1A antibody [EPR11790(B)]
See all KIF1A primary antibodies
Description

Rabbit monoclonal [EPR11790(B)] to KIF1A
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human KIF1A aa 900-1000. The exact sequence is proprietary.
Database link: Q12756
Positive control
- HEK-293 cells, Human fetal brain lysates, SH-SY5Y cells, U-87 MG cells, rat hypothalamus lysate and rat brain lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR11790(B)
Isotype

IgG
Research areas

Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

All lanes : Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/1000 dilution (purified)

Lane 1 : Rat hypothalamus lysate
Lane 2 : Rat brain lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 191 kDa
Observed band size: 191 kDa

Blocking and diluting buffer and concentration: 5% NFDM /TBST.
Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling KIF1A with purified ab180153 at 1/100 dilution (9.2μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilised with 0.1% tritonX-100. Ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used as the secondary antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as the nuclear counter stain.
Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

ab180153 (purified) at 1/50 dilution (2ug) immunoprecipitating KIF1A in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate.

Lane 1 (input): SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 10ug

Lane 2 (+): ab180153 + SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 10ug

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab180153 in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

For western blotting, ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection (1/10000).

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.
Flow Cytometry - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling KIF1A with purified ab180153 at 1/90 dilution (10 ug/ml). Cells were fixed with 4% paraformaldehyde. A goat anti-rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/2000 dilution. Black - Isotype control, Rabbit monoclonal IgG. Blue - unlabelled control, cells without incubation with primary antibody and secondary antibody.
Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/10000 dilution (purified) + HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 191 kDa
Observed band size: 191 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling KIF1A with unpurified ab180153 at 1/100 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counter stained with Dapi.
Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/20000 dilution (unpurified) + 293 cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 191 kDa
Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Western blot analysis of 293 cell lysate immunoprecipitated using unpurified ab180153 at 1/30 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling KIF1A with unpurified ab180153 at 1/150 dilution (red), compared to a Rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.
Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/10000 dilution (unpurified) + Human fetal brain lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 191 kDa
Anti-KIF1A antibody [EPR11790(B)] (ab180153)

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