Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GATA4 antibody [EPR23691-12] (ab256782)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23691-12] to GATA4
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-GATA4 antibody [EPR23691-12]
    See all GATA4 primary antibodies

  • Description

    Rabbit monoclonal [EPR23691-12] to GATA4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, Huh7 and HEK-293T whole cell lysate. Human liver cancer tissue lysate. IP: Huh7 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-GATA4 antibody [EPR23691-12] (ab256782)
    Western blot - Anti-GATA4 antibody [EPR23691-12] (ab256782)
    All lanes : Anti-GATA4 antibody [EPR23691-12] (ab256782) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg
    Lane 2 : Huh7 (human hepatocellar carcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), whole cell lysate at 20 µg
    Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The lane 1 of blot was developed using a higher sensitivity ECL substrate. 

    The molecular weight observed is consistent with what has been described in the literature (PMID: 28758902, 16940177, 18426912, 31903133). 

    20 kDa band in HepG2 whole cell lysate is consistent with what has been described in the literature (PMID: 25501827). 

    Negative control: HeLa (PMID: 24696446, 24352556, 31322241)

    Exposure time: Lane 1: 101 seconds; Lane 2-4: 3 minutes.

  • Immunoprecipitation - Anti-GATA4 antibody [EPR23691-12] (ab256782)
    Immunoprecipitation - Anti-GATA4 antibody [EPR23691-12] (ab256782)

    GATA4 was immunoprecipitated from 0.35 mg Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 µg with ab256782 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256782 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Huh7 whole cell lysate 10 µg.

    Lane 2: ab256782 IP in Huh7 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256782 in Huh7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 49 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 31903133). 20 kDa band in Huh7 whole cell lysate is consistent with what has been described in the literature (PMID: 25501827).

  • Western blot - Anti-GATA4 antibody [EPR23691-12] (ab256782)
    Western blot - Anti-GATA4 antibody [EPR23691-12] (ab256782)
    Anti-GATA4 antibody [EPR23691-12] (ab256782) at 1/1000 dilution + Human liver cancer tissue lysate at 20 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 44 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-GATA4 antibody [EPR23691-12] (ab256782)
    Anti-GATA4 antibody [EPR23691-12] (ab256782)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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