Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR102H] to ZEB1
Suitable for: WB, IP
Reacts with: Mouse, Human

Product name

Anti-ZEB1 antibody [BLR102H]
See all ZEB1 primary antibodies
Description

Rabbit monoclonal [BLR102H] to ZEB1
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide within Human ZEB1 aa 1074-1124 (C terminal). The exact sequence is proprietary.
Database link: P37275
Positive control
- WB: Jurkat and RKO cells; C2C12, 70Z/3, BW5147.3, EL4, RenCa, NIH 3T3, TCMK-1, A20, Ba/F3, and CH27 cells. IP: HEK-293T cells.
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Application	Species
IP	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 8.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 98% Borate buffered saline
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

BLR102H
Isotype

IgG
Research areas

Immunoprecipitation - Anti-ZEB1 antibody [BLR102H] (ab276129)

ZEB1 was immunoprecipitated from 20 µl/mg HEK-293T whole cell lysate, prepared using NETN lysis buffer. ab276129 was used for IP at 1:1000 (Lane 1). Control IgG (Lane 2).

Chemiluminescence with an exposure time of 30 seconds.
Western blot - Anti-ZEB1 antibody [BLR102H] (ab276129)

All lanes : Anti-ZEB1 antibody [BLR102H] (ab276129) at 1/1000 dilution

Lane 1 : C2C12 whole cell lysate
Lane 2 : 70Z/3 whole cell lysate
Lane 3 : BW5147.3 whole cell lysate
Lane 4 : CTLL-2 whole cell lysate
Lane 5 : EL4 whole cell lysate
Lane 6 : RenCa whole cell lysate
Lane 7 : NIH/3T3 whole cell lysate
Lane 8 : TCMK-1 whole cell lysate
Lane 9 : A20 whole cell lysate
Lane 10 : Ba/F3 whole cell lysate
Lane 11 : CH27 whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Predicted band size: 124 kDa

Chemiluminescence with an exposure time of 30 seconds.

Lower Panel: Rabbit anti-COPB2 antibody.
Western blot - Anti-ZEB1 antibody [BLR102H] (ab276129)

All lanes : Anti-ZEB1 antibody [BLR102H] (ab276129) at 1/1000 dilution

Lane 1 : GaMG whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : HEK-293T whole cell lysate
Lane 4 : RKO whole cell lysate
Lane 5 : MCF-7 whole cell lysate
Lane 6 : A549 whole cell lysate
Lane 7 : K562 whole cell lysate
Lane 8 : HeLa whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Predicted band size: 124 kDa

Chemiluminescence with an exposure time of 30 seconds.

Lower Panel: Rabbit anti-COPB2 antibody
Anti-ZEB1 antibody [BLR102H] (ab276129)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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