Anti-ZEB1 antibody [BLR102H] (ab276129)
![Anti-ZEB1 antibody [BLR102H] (ab276129)](https://www.abcam.com/ps/products/276/ab276129/Images/ab276129-412238-AntiZEB1antibodyBLR102Himmunoprecipitation293Tcells.png "Anti-ZEB1 antibody [BLR102H] (ab276129)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR102H] to ZEB1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-ZEB1 antibody [BLR102H]
See all ZEB1 primary antibodies -
Description
Rabbit monoclonal [BLR102H] to ZEB1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human ZEB1 aa 1074-1124 (C terminal). The exact sequence is proprietary.
Database link: P37275 -
Positive control
- WB: Jurkat and RKO cells; C2C12, 70Z/3, BW5147.3, EL4, RenCa, NIH 3T3, TCMK-1, A20, Ba/F3, and CH27 cells. IP: HEK-293T cells.
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 98% Borate buffered saline -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
BLR102H -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-ZEB1 antibody [BLR102H] (ab276129)
ZEB1 was immunoprecipitated from 20 µl/mg HEK-293T whole cell lysate, prepared using NETN lysis buffer. ab276129 was used for IP at 1:1000 (Lane 1). Control IgG (Lane 2).
Chemiluminescence with an exposure time of 30 seconds.
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![Western blot - Anti-ZEB1 antibody [BLR102H] (ab276129)](https://www.abcam.com/ps/products/276/ab276129/Images/ab276129-412237-AntiZEB1-antibody-BLR102H-westernblot--C2C12-70Z-3-BW51473-CTLL2-EL4-RenCa-NIH-3T3-TCMK1-A20-Ba-F3-and-CH27-cells.JPG)
Western blot - Anti-ZEB1 antibody [BLR102H] (ab276129)All lanes : Anti-ZEB1 antibody [BLR102H] (ab276129) at 1/1000 dilution
Lane 1 : C2C12 whole cell lysate
Lane 2 : 70Z/3 whole cell lysate
Lane 3 : BW5147.3 whole cell lysate
Lane 4 : CTLL-2 whole cell lysate
Lane 5 : EL4 whole cell lysate
Lane 6 : RenCa whole cell lysate
Lane 7 : NIH/3T3 whole cell lysate
Lane 8 : TCMK-1 whole cell lysate
Lane 9 : A20 whole cell lysate
Lane 10 : Ba/F3 whole cell lysate
Lane 11 : CH27 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 124 kDaChemiluminescence with an exposure time of 30 seconds.
Lower Panel: Rabbit anti-COPB2 antibody.
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![Western blot - Anti-ZEB1 antibody [BLR102H] (ab276129)](https://www.abcam.com/ps/products/276/ab276129/Images/ab276129-412236-AntiZEB1-antibody-BLR102H-westernblot.JPG)
Western blot - Anti-ZEB1 antibody [BLR102H] (ab276129)All lanes : Anti-ZEB1 antibody [BLR102H] (ab276129) at 1/1000 dilution
Lane 1 : GaMG whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : HEK-293T whole cell lysate
Lane 4 : RKO whole cell lysate
Lane 5 : MCF-7 whole cell lysate
Lane 6 : A549 whole cell lysate
Lane 7 : K562 whole cell lysate
Lane 8 : HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 124 kDaChemiluminescence with an exposure time of 30 seconds.
Lower Panel: Rabbit anti-COPB2 antibody
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![Anti-ZEB1 antibody [BLR102H] (ab276129)](https://www.abcam.com/ps/products/276/ab276129/Images/ab276129-4-benefits-of-recombinant-antibodies.png)
Anti-ZEB1 antibody [BLR102H] (ab276129)