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Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15826] to ACF1 / BAZ1A - C-terminal
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal
    See all ACF1 / BAZ1A primary antibodies

  • Description

    Rabbit monoclonal [EPR15826] to ACF1 / BAZ1A - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Daudi, Molt4 and Jurkat whole cell lysate (ab7899). IHC-P: Human colon and Human testis tissues. ICC/IF: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15826

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)
    Western blot - Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)
    All lanes : Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670) at 1/10000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : BAZ1A knockout cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 178 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab187670 observed at 178 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab187670 Anti-BAZ1A antibody [EPR15826] - C-terminal was shown to specifically react with BAZ1A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264925 (knockout cell lysate ab257856) was used. Wild-type and BAZ1A knockout samples were subjected to SDS-PAGE. ab187670 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labeling ACF1 / BAZ1A with ab187670 at 1/100 dilution. Prediluted (Ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Antigen retrieval: Perform heat mediated antigen retrieval using EDTA Buffer, pH 9. Counter stain: Hematoxylin.

  • Western blot - Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)
    Western blot - Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)
    All lanes : Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670) at 1/10000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : BAZ1A knockout HAP1 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 178 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab187670 observed at 178 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab187670 was shown to specifically react with BAZ1A in wilt-type HAP1 cells. No band was observed when BAZ1A knockout samples were used. Wild-type and BAZ1A knockout samples were subjected to SDS-PAGE.  Ab187670 and ab18058 (loading control to Vinculin) were diluted at 1/10000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)
    Western blot - Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)
    All lanes : Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Molt4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 178 kDa

  • Western blot - Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)
    Western blot - Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)
    Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670) at 1/50000 dilution + Jurkat cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 178 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)

    Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ACF1 / BAZ1A with ab187670 at 1/100 dilution. Prediluted (Ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Antigen retrieval: Perform heat mediated antigen retrieval using EDTA Buffer, pH 9. Counter stain: Hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)
    Immunocytochemistry/ Immunofluorescence - Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling ACF1 / BAZ1A with ab187670 at 1/250 (red) and DAPI staining (blue). Secondary aantibody: goat anti rabbit IgG(Alexa Fluor® 555).

  • Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)
    Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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