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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Recombinant Human Egr1 protein (ab185397)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE, HPLC

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Description

  • Product name

    Recombinant Human Egr1 protein
  • Purity

    > 95 % SDS-PAGE.
    Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
  • Endotoxin level

  • Expression system

    Escherichia coli
  • Accession

    P18146
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHHSSGLVPRGSHMQQPSLTPLSTIKAFATQSGSQDLKALNTS YQSQLIKPSRMRKYPNRPSKTPPHERPYACPVESCDRRFSRSDELTRHIR IHTGQKPFQCRICMRNFSRSDHLTTHIRTHTGEKPFACDICGRKFARSDE RKRHTKIHLRQKDKKADKSVVAS
    • Predicted molecular weight

      20 kDa including tags
    • Amino acids

      282 to 433
    • Tags

      His tag N-Terminus
  • Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab185397 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      HPLC

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Reconstitute for long term storage.

      pH: 7.40
      Constituent: 100% PBS

    • Reconstitution
      Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 3X PBS. Please aliquot the reconstituted solution

    General Info

    • Alternative names

      • AT225
      • Early growth response 1
      • Early growth response protein 1
      • EGR 1
      • EGR-1
      • EGR1
      • EGR1_HUMAN
      • G0S30
      • KROX 24
      • KROX24
      • Nerve growth factor-induced clone A
      • Nerve growth factor-induced protein A
      • NGFI-A
      • NGFIA
      • TIS8
      • Transcription factor ETR103
      • Transcription factor Zif268
      • ZIF 268
      • ZIF268
      • Zinc finger protein 225
      • Zinc finger protein Krox-24
      • ZNF225
      see all
    • Function

      Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
    • Sequence similarities

      Belongs to the EGR C2H2-type zinc-finger protein family.
      Contains 3 C2H2-type zinc fingers.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P18146 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab185397? Please let us know so that we can cite the reference in this datasheet.

    ab185397 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Reconstitute for long term storage.

      pH: 7.40
      Constituent: 100% PBS

    • Reconstitution
      Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 3X PBS. Please aliquot the reconstituted solution

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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