Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP529Y] to Bcl6
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-Bcl6 antibody [EP529Y]
See all Bcl6 primary antibodies
Description

Rabbit monoclonal [EP529Y] to Bcl6
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Bcl6 aa 650-750 (C terminal). The exact sequence is proprietary.
Positive control
- WB: Daudi and Ramos, HeLa and SH-SY5Y cell lysates.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP529Y
Isotype

IgG
Research areas

Western blot - Anti-Bcl6 antibody [EP529Y] (ab33901)

All lanes : Anti-Bcl6 antibody [EP529Y] (ab33901) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BCL6 knockout HeLa cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : SH-SY5Y cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 78 kDa
Observed band size: 78 kDa

Lanes 1-4: Merged signal (red and green). Green ab33901 observed at 78 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab33901 Anti-Bcl6 antibody [EP529Y] was shown to react with BCL6 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265410 (knockout cell lysate ab257178) was used. Wild-type and Bcl6 knockout samples were subjected to SDS-PAGE. ab33901 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Bcl6 antibody [EP529Y] (ab33901)

All lanes : Anti-Bcl6 antibody [EP529Y] (ab33901) at 1/1000 dilution (Purified)

Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method
Lane 2 : Ramos (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 78 kDa

Exposure time: 10 seconds

Blocking and diluting buffer: 5% NFDM/TBST.

This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
Western blot - Anti-Bcl6 antibody [EP529Y] (ab33901)

All lanes : Anti-Bcl6 antibody [EP529Y] (ab33901) at 1/1000 dilution (Purified)

Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in RIPA lysis method
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 78 kDa

Exposure time: 3 minutes

Blocking and diluting buffer: 5% NFDM/TBST.

This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol here (downloadable copy).
Western blot - Anti-Bcl6 antibody [EP529Y] (ab33901)

Anti-Bcl6 antibody [EP529Y] (ab33901) at 1/2000 dilution (Unpurified) + Daudi cell lysate

Predicted band size: 78 kDa
Observed band size: 85-90 kDa
why is the actual band size different from the predicted?
Anti-Bcl6 antibody [EP529Y] (ab33901)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com