Anti-Zfp219 antibody (ab31003)
Key features and details
- Rabbit polyclonal to Zfp219
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Zfp219 antibody -
Description
Rabbit polyclonal to Zfp219 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse Zfp219 aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab31002) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: F9; IOUD2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RatHumanApplication Abreviews Notes WB (2) 1/250. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).Notes WB
1/250. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).Target
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Function
May be involved in transcriptional regulation. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 51222 Human
- Entrez Gene: 69890 Mouse
- Entrez Gene: 305848 Rat
- SwissProt: Q9P2Y4 Human
- SwissProt: Q4VA98 Mouse
- SwissProt: Q66H48 Rat
- Unigene: 250493 Human
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Alternative names
- Zinc finger protein 219 antibody
- ZN219_HUMAN antibody
- ZNF219 antibody
Images
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Western blot - Anti-Zfp219 antibody (ab31003)All lanes : Anti-Zfp219 antibody (ab31003) at 1/250 dilution
Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 2 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate
Lysates/proteins at 20 µg/ml per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDa
Additional bands at: 58 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Datasheets and documents
References (0)
ab31003 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Zfp219 antibody (ab31003)All lanes : Anti-Zfp219 antibody (ab31003) at 1/250 dilution
Lane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 2 : IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate
Lysates/proteins at 20 µg/ml per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDa
Additional bands at: 58 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes