Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CTCF antibody [BLR041F] - BSA free (ab272091)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR041F] to CTCF - BSA free
Suitable for: ICC, IHC-P, IP
Reacts with: Mouse, Human

Product name

Anti-CTCF antibody [BLR041F] - BSA free
See all CTCF primary antibodies
Description

Rabbit monoclonal [BLR041F] to CTCF - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Mouse Human
IP	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CTCF aa 650-700. The exact sequence is proprietary. NP_006556.1 and Gene ID 10664.
Database link: P49711
General notes

This product is sold under License from Bethyl Laboratories, Inc.

ab272091 is the carrier-free version of ab243897. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR041F
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [BLR041F] - BSA free (ab272091)

Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for CTCF using ab243897 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243897).
Immunocytochemistry - Anti-CTCF antibody [BLR041F] - BSA free (ab272091)

Formalin-fixed, paraffin-embedded MCF7 (human breast adenocarcinoma cell line) cells labeling CTCF using ab243897 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243897).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [BLR041F] - BSA free (ab272091)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for CTCF using ab243897 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243897).
Immunoprecipitation - Anti-CTCF antibody [BLR041F] - BSA free (ab272091)

CTCF was immunoprecipitated from 1.0 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab243897 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243897 at 1/1000 dilution.

Lane 1: ab243897 IP in HEK-293T whole cell lysate.

Lane 2: Control IgG in HEK-293T whole cell lysate.

Detection: Chemiluminescence with an exposure time of 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243897).
Anti-CTCF antibody [BLR041F] - BSA free (ab272091)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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