PPAR (alpha, delta, gamma) Transcription Factor Assay Kit (ab133113)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Nuclear Extracts, Suspension cells
Overview
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Product name
PPAR (alpha, delta, gamma) Transcription Factor Assay Kit -
Detection method
Colorimetric -
Sample type
Adherent cells, Suspension cells, Nuclear Extracts -
Assay type
Semi-quantitative -
Species reactivity
Reacts with: Human
Predicted to work with: Mammals
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Product overview
Abcam's PPAR Transcription Factor Assay Kit (ab133113) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the PPAR response element is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPAR response element. PPAR alpha, delta, or gamma are detected by addition of specific primary antibodies directed against the individual PPARs. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. ab133113 comes with a single plate that measures all three isoforms of PPAR alpha, delta, and gamma. There are enough reagents for one-third of a plate for each isoform.
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Notes
Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands. PPARs play pivotal roles in the regulation of lipid metabolism and homeostasis and are important indirect and direct regulators of cellular insulin sensitivity.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 96 tests 96-Well Plate Cover 1 unit Polysorbate 20 1 vial Transcription Factor Antibody Binding Buffer (10X) 1 x 3ml Transcription Factor Binding Assay Buffer (4X) 1 x 3ml Transcription Factor Complete PPARα Positive Control 1 vial Transcription Factor Complete PPARα Primary Antibody 1 vial Transcription Factor Complete PPARδ Positive Control 1 vial Transcription Factor Complete PPARδ Primary Antibody 1 vial Transcription Factor Complete PPARγ Positive Control 1 vial Transcription Factor Complete PPARγ Primary Antibody 1 vial Transcription Factor Developing Solution 1 x 12ml Transcription Factor Goat Anti-Rabbit HRP Conjugate 1 x 100µl Transcription Factor PPAR 96-Well Strip Plate 1 unit Transcription Factor PPAR Competitor dsDNA 1 vial Transcription Factor Reagent A 1 x 120µl Transcription Factor Stop Solution 1 x 12ml Wash Buffer Concentrate (400X) 1 x 5ml -
Research areas
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Cellular localization
Cytoplasm, nucleus. -
Alternative names
- CIMT1
- FAAR
- GLM1
see all -
Database links
- Entrez Gene: 5465 Human
- Entrez Gene: 5467 Human
- Entrez Gene: 5468 Human
- Omim: 600409 Human
- Omim: 170998 Human
- SwissProt: Q07869 Human
- SwissProt: P37231 Human
- SwissProt: Q03181 Human
Images
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Transcription Factor Binding Assay for PPAR gammaIncreasing amounts of positive control (total lysate) are assayed for PPAR gamma DNA-binding activity using ab133113.
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Transcription Factor Binding Assay for PPAR deltaIncreasing amounts of positive control (total lysate) are assayed for PPAR delta DNA-binding activity using ab133113. -
Transcription Factor Binding Assay for PPAR alphaIncreasing amounts of positive control (total lysate) are assayed for PPAR alpha DNA-binding activity using ab133113.
