Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant human IL-27-A + EBI3 protein (Fc Chimera Active) (ab214982)

Key features and details

  • Expression system: CHO cells
  • Purity: >= 98% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human IL-27-A + EBI3 protein (Fc Chimera Active)

  • Biological activity

    Bioactivity was measured in a cell proliferation assay using anti-CD3 activated human peripheral mononuclear cells.

  • Purity

    >= 98 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    CHO cells

  • Accession

    Q8NEV9
    Q14213

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Amino Acid Sequence 1

    • Species

      Human

    • Sequence

      VWGFPRPPGRPQLSLQELRREFTVSLHLARKLLSEVRGQAHRFAESHLPG VNLYLLPLGEQLPDVSLTFQAWRRLSDPERLCFISTTLQPFHALLGGLGT QGRWTNMERMQLWAMRLDLRDLQRHLRFQVLAAGFNLPEEEEEEEEEEEE ERKGLLPGALGSALQGPAQVSWPQLLSTYRLLHSLELVLSRAVRELLLLS KAGHSVWPLGFPTLSPQP

    • Amino acids

      26 to 243

    • Additional sequence information

      Mature protein without signal peptide fused through a polypeptide linker to the Fc portion of a mutant Human IgG1.

    • Amino Acid Sequence 2

    • Species

      Human

    • Sequence

      RKGPPAALTLPRVQCRASRYPIAVDCSWTLPPAPNSTSPVSFIATYRLGM AARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVLNVTAVHPWGSSSSFVP FITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSLKYWIRYK RQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLP ATATMSLGK

    • Amino acids

      21 to 229

    • Additional sequence information

      Mature protein without signal peptide fused through a polypeptide linker to the Fc portion of a mutant human IgG1.

    • Specifications

    Our Abpromise guarantee covers the use of ab214982 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

    • Form

      Lyophilized

    • Additional notes

      Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.

      Constituent: 100% PBS

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Reconstitute in 50 µL sterile water. Add 1X PBS to the desired protein concentration. Working aliquots are stable for up to 3 months when stored at -20°C.

    General Info

    • Alternative names

      • IL27-A+EBI3

    • Cellular localization

      IL27-A: Secreted. Does not seem to be secreted without coexpression of EBI3. EBI3: Secreted.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab214982? Please let us know so that we can cite the reference in this datasheet.

ab214982 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Alternative names

    • IL27-A+EBI3

  • Cellular localization

    IL27-A: Secreted. Does not seem to be secreted without coexpression of EBI3. EBI3: Secreted.

Images

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