Recombinant human IL-27-A+EBI3 protein (Active) (ab245785)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC
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Product name
Recombinant human IL-27-A+EBI3 protein (Active) -
Biological activity
Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 range is 400-500 ng/ml.
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Purity
> 90 % SDS-PAGE.
>90% by HPLC. -
Endotoxin level
Expression system
HEK 293 cellsAccession
Protein length
Full length proteinAnimal free
NoNature
Recombinant-
Amino Acid Sequence 1
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Species
Human -
Sequence
FPRPPGRPQLSLQELRREFTVSLHLARKLLSEVRGQAHRFAESHLPGVNL YLLPLGEQLPDVSLTFQAWRRLSDPERLCFISTTLQPFHALLGGLGTQGR WTNMERMQLWAMRLDLRDLQRHLRFQVLAAGFNLPEEEEEEEEEEEEERK GLLPGALGSALQGPAQVSWPQLLSTYRLLHSLELVLSRAVRELLLLSKAG HSVWPLGFPTLSPQP -
Predicted molecular weight
25 kDa -
Amino acids
29 to 243 -
Additional sequence information
Full-length mature chain lacking the signal peptide.
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Amino Acid Sequence 2
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Species
Human -
Sequence
RKGPPAALTLPRVQCRASRYPIAVDCSWTLPPAPNSTSPVSFIATYRLGM AARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVLNVTAVHPWGSSSSFVP FITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSLKYWIRYK RQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLP ATATMSLGK -
Amino acids
21 to 229 -
Additional sequence information
Full-length mature chain lacking the signal peptide
Associated products
Specifications
Our Abpromise guarantee covers the use of ab245785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
HPLC
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Form
Lyophilized -
Additional notes
ab245785 is a non-disulfide-linked, heterodimeric protein composed of a 209 amino acid length EBI3 subunit and a 215 amino acid length IL-27-p28 subunit, for a total sequence length of 424 amino acid residues. The calculated molecular weight of the associated IL-27 subunits is 47.8 kDa.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Sterile filtered through a 0.2 micron filter. Lyophilized from 10mM Sodium Phosphate, pH 6.5 + 100mM
NaCl.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge vial prior to opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex. Store at 2°C to 8°C for 1 week, or prepare for extended storage.
General Info
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Alternative names
- IL27-A+EBI3
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Cellular localization
IL27-A: Secreted. Does not seem to be secreted without coexpression of EBI3. EBI3: Secreted.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab245785 has not yet been referenced specifically in any publications.
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Sterile filtered through a 0.2 micron filter. Lyophilized from 10mM Sodium Phosphate, pH 6.5 + 100mM
NaCl.This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionCentrifuge vial prior to opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex. Store at 2°C to 8°C for 1 week, or prepare for extended storage.
