Mouse IL-3 ELISA Kit (ab222509)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 12 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
-
Product name
Mouse IL-3 ELISA Kit
See all IL-3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Standard 5 5.9% Inter-assay Sample n Mean SD CV% Standard 3 4.3% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
12 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 102 99% - 107% Cell culture media 103 96% - 113% Hep Plasma 115 109% - 123% EDTA Plasma 99 90% - 107% Cit plasma 106 103% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IL-3 ELISA Kit (ab222509) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-3 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-3 with 12 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Interleukin 3 (IL-3) is a secreted 166 amino acids long, glycosylated interleukin coded by the IL3 gene. Previous names reported for IL-3 are Hematopoietic growth factor, Mast cell growth factor, multi-potential colony-stimulating factor, and P-cell-stimulating factor. IL-3 has been found to stimulate a variety of cell activities such as; proliferation, differentiation, survival of pluripotent hematopoietic stem cells, and may be associated with neurologic disorders. IL-3 is expressed by activated T-cells, mast cells, and natural killer cells. IL-3 performs its biological action by binding to a transmembrane receptor complex consisting of IL-3RA, also known as CD123, and a signal inducing beta subunit, which also acts as a receptor for colony stimulating factor 2 and Interleukin 5.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse IL-3 Capture Antibody 1 x 600µl 10X Mouse IL-3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse IL-3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Sample Diluent NS (ab193972) 1 x 50ml Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. -
Tissue specificity
Activated T-cells, mast cells, natural killer cells. -
Sequence similarities
Belongs to the IL-3 family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- Colony stimulating factor multiple
- Hematopoietic growth factor
- IL 3
see all -
Database links
- Entrez Gene: 16187 Mouse
- SwissProt: P01586 Mouse
- Unigene: 983 Mouse
Images
-
Other - Mouse IL-3 ELISA Kit (ab222509)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of mouse IL-3 standard curveThe IL-3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of spiked IL-3 in mouse serum, and plasma samplesThe concentrations of IL-3 were measured in duplicates, interpolated from the IL-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
-
Interpolated concentrations of native IL-3 in an unstimulated and PHAM (10 µg/mL PHA and 10 ng/mL PMA, 48 hr) stimulated EL4 cell culture supernatant samplesThe concentrations of IL-3 were measured in duplicate and interpolated from the IL-3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-3 concentration was determined to be 1834 pg/mL in neat stimulated EL4 culture supernatant samples and not detectable in unstimulated samples.
-
Sandwich ELISA - Mouse IL-3 ELISA Kit (ab222509)To learn more about the advantages of recombinant antibodies see here.
