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Human Coronin 1a ELISA Kit (ab214032)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 49 pg/ml
  • Range: 0.313 ng/ml - 20 ng/ml
  • Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Coronin 1a ELISA Kit
    See all Coronin 1a/TACO kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Human serum 5 5.6%
    Inter-assay
    Sample n Mean SD CV%
    Human serum 3 6.4%

  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    49 pg/ml

  • Range

    0.313 ng/ml - 20 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 93 89% - 98%
    Cell culture extracts 102 80% - 120%
    Hep Plasma 96 93% - 98%
    EDTA Plasma 93 89% - 94%
    Cit plasma 83 79% - 87%
    serum free media 94 88% - 98%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Coronin 1a ELISA Kit (ab214032) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Coronin 1a protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Coronin 1a with 49 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Coronin 1A is a WD-repeat containing protein that plays a role in cytoskeleton dynamics. Coronin 1A binds to actin. Mouse and rat Coronin 1A have 95% and 93% protein sequence identity to human Coronin 1A.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Coronin 1a Capture Antibody 1 x 600µl
    10X Human Coronin 1a Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BI 1 x 6ml
    Human Coronin 1a Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes.

  • Tissue specificity

    Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut.

  • Sequence similarities

    Belongs to the WD repeat coronin family.
    Contains 7 WD repeats.

  • Cellular localization

    Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle > phagosome membrane. In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification.
  • Information by UniProt

  • Alternative names

    • Actin binding protein
    • CLABP
    • Clipin A
    • Clipin-A
    • CLIPINA
    • COR1A_HUMAN
    • CORO1A
    • Coronin 1
    • Coronin actin binding protein 1A
    • Coronin like protein A
    • Coronin like protein p57
    • Coronin-1A
    • Coronin-like protein A
    • Coronin-like protein p57
    • FLJ41407
    • HCORO1
    • MGC117380
    • OTTHUMP00000163017
    • p57
    • TACO
    • Tryptophan aspartate containing coat protein
    • Tryptophan aspartate-containing coat protein
    see all

  • Database links

    Images

    • Other - Human Coronin 1a ELISA Kit (ab214032)
      Other - Human Coronin 1a ELISA Kit (ab214032)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of Human Coronin 1a standard curve in Sample Diluent NS.
      Example of Human Coronin 1a standard curve in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of Human Coronin 1a standard curve in 1X Cell Extraction Buffer PTR.
      Example of Human Coronin 1a standard curve in 1X Cell Extraction Buffer PTR.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Serum from ten individual healthy human female donors was measured in duplicate.
      Serum from ten individual healthy human female donors was measured in duplicate.

      Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Coronin 1a concentration was determined to be 85.97 ng/mL with a range of 4.85 – 716.8 ng/mL.

    • Interpolated concentrations of native Coronin 1a in human HEPG2, MCF7, and Jurkat cell extract samples and samples based on a 400 µg/mL, 200 µg/mL, and 8 µg/mL extract load respectively
      Interpolated concentrations of native Coronin 1a in human HEPG2, MCF7, and Jurkat cell extract samples and samples based on a 400 µg/mL, 200 µg/mL, and 8 µg/mL extract load respectively

      The concentrations of Coronin 1a were measured in duplicate and interpolated from the Coronin 1a standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Coronin 1a concentration was determined to be 21.4 pg/µg HEPG2 extract, 54.4 pg/ µg MCF7 extract and 888.5 pg /µg Jurkat extract.

    • Interpolated concentrations of native Coronin 1a in human Peripheral Blood Monocyte Cell (PBMC) culture supernatant samples
      Interpolated concentrations of native Coronin 1a in human Peripheral Blood Monocyte Cell (PBMC) culture supernatant samples

      PBMC cells were grown in the absence (unstimulated) or presence of Phorbol Myistrate Acetate (PMA and phytohemagglutinin (PHA) (stimulated) for 3 days. The concentrations of Coronin 1a were measured in duplicates, interpolated from the Coronin 1a standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated 50% and stimulated (25%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Coronin 1a concentration was determined to be 38.7 ng/mL in unstimulated and 76.9 ng/mL in stimulated, and 6.7ng/mL in media. This pair is highly cross reactive with bovine serum. Dilute bovine serum containing media such that the concentration of bovine serum is less than 0.1% or run a media control sample.

    • Sandwich ELISA - Human Coronin 1a ELISA Kit (ab214032)
      Sandwich ELISA - Human Coronin 1a ELISA Kit (ab214032)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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