Human Coronin 1a Matched Antibody Pair Kit (ab216793)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 39.1 pg/ml - 2500 pg/ml
Overview
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Product name
Human Coronin 1a Matched Antibody Pair Kit
See all Coronin 1a/TACO kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
10.2 pg/ml -
Range
39.1 pg/ml - 2500 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Coronin 1a Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Coronin 1a.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab214032. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Coronin 1a Capture Antibody 2 x 50µg 1 x 50µg Human Coronin 1a Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Coronin 1a Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. -
Tissue specificity
Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut. -
Sequence similarities
Belongs to the WD repeat coronin family.
Contains 7 WD repeats. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle > phagosome membrane. In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification. - Information by UniProt
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Alternative names
- Actin binding protein
- CLABP
- Clipin A
see all -
Database links
- Entrez Gene: 11151 Human
- Omim: 605000 Human
- SwissProt: P31146 Human
- Unigene: 415067 Human
Images
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Human Coronin 1a standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human Coronin 1a Matched Antibody Pair Kit (ab216793)To learn more about the advantages of recombinant antibodies see here.
